Category: Annexin Binding Buffer
Electrophoretic transfer of proteins from polyacrylamide
Electrophoretic switch of proteins from polyacrylamide gels to nitrocellulose sheets: process and a few purposes. A way has been devised for the electrophoretic switch of proteins from polyacrylamide gels to nitrocellulose sheets. The strategy ends in quantitative switch of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the unique band sample was…
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Graduate training programs in pathology
PhD graduate coaching applications in pathology: 2003 report from the American Society for Investigative Pathology workshop on pathology graduate schooling. Graduate coaching is likely one of the most necessary actions wherein we interact. By PhD coaching applications in pathology, we regularly renew the mental vigor of our self-discipline and guarantee its place as a vibrant power within the life sciences.…
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First EUROPEAN PhD and Post-Doc Symposium
ENABLE 2017, the First EUROPEAN PhD and Publish-Doc Symposium. Session 4: From Discovery to Treatment: The Way forward for Therapeutics. The EUROPEAN ACADEMY FOR BIOMEDICAL SCIENCE (ENABLE) is an initiative funded by the European Union Horizon 2020 program involving 4 famend European Analysis Institutes (Institute for Analysis in Biomedicine-IRB Barcelona, Spain; Radboud Institute for Molecular Life Sciences-RIMLS, the Netherlands; Novo Nordisk…
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β-Defensins from common goby (Pomatoschistus microps) and silver trevally (Pseudocaranx georgianus): Molecular characterization and phylogenetic analysis
Antimicrobial peptides (AMPs) are biologically active molecules involved in host defense present in a variety of organisms. They are an integral component of innate immunity, forming a front line of defense against potential pathogens, including antibiotic-resistant ones. Fishes are proven to be a prospective source of AMPs as they are constantly being challenged by a…
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MOLECULAR INVESTIGATION AND PHYLOGENETIC ANALYSIS OF ANAPLASMOSIS IN DOGS
Anaplasmosis is a widespread vector-borne illness affecting canines, and Anaplasma platys is the key etiological agent of the illness. The research examines anaplasmosis molecular prevalence, associated threat elements, and alteration of hematological variables in Anaplasma-affected canines. A complete of 150 blood samples have been collected from canines within the district of Lahore, Pakistan. The…
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First report of molecular and phylogenetic evaluation of Physaloptera praeputialis in naturally contaminated stray cats from India Nematodes of the genus Physaloptera are globally distributed and infect a large number of hosts. Their life cycle includes orthopterans and coleopterans as intermediate hosts. The morphological characters alone are insufficient to detect and differentiate Physaloptera spp. from its congeners. Furthermore,…
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An overview of molecular biology and nanotechnology based analytical methods for the detection of SARS-CoV-2: promising biotools for the rapid diagnosis of COVID-19
At present, the 2019 novel coronavirus (2019-nCoV) is drastically affecting 214 nations, inflicting extreme pneumonia in sufferers, which has resulted in lockdown being applied in a number of nations to cease its native transmission. Contemplating this, the fast screening and correct detection of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2; 2019-nCoV) play a vital position…
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Peak force tapping atomic force microscopy for advancing cell and molecular biology
The appearance of atomic drive microscopy (AFM) offers an thrilling device to detect molecular and mobile behaviors underneath aqueous situations. AFM is ready to not solely visualize the floor topography of the specimens, but additionally can quantify the mechanical properties of the specimens by drive spectroscopy assay. However, integrating AFM topographic imaging with drive spectroscopy…
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Molecular Biology Methods in Streptomyces rimosus, a Producer of Oxytetracycline
Streptomyces rimosus is used for manufacturing of the broad-spectrum antibiotic oxytetracycline (OTC). S. rimosus belongs to Actinomyces species, a big group of microorganisms that produce various set of pure metabolites of excessive significance in lots of elements of our life. On this chapter, we describe particular molecular biology strategies and a classical homologous recombination strategy…
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Lyophilization and homogenization of biological samples improves reproducibility and reduces standard deviation in molecular biology techniques
Lyophilization is an economical technique for organic specimen preservation however detailed tissue-specific reference protocols are nonetheless missing. Furthermore, knowledge are restricted on the long-term stability of proteins and nucleic acids in lyophilized samples. Right here, we provide lyophilization protocols for varied rat and mouse tissues (kidney, coronary heart, liver, lung, aorta, and pores and skin)…
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