BioHazards Bio-informe Fundamental Tools

Background Information
Chromatin immunoprecipitaton (ChIP) provides an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions could possibly be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could possibly be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Package deal is an entire set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle may be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

• Terribly fast course of: your whole course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
• Helpful and versatile: the bundle is suitable for getting ready every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
• Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

Principle & Course of
The ChromaFlash™ Chromatin Extraction Package deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, modern and frozen tissues, and so forth. The amount of cells and tissues for each preparation could possibly be 1 x 10⁵ to 5 x 10⁶ cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 10⁶ cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 10⁶  cells or per 50 mg tissues.

Background Information
Chromatin immunoprecipitation (ChIP) provides an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions could possibly be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could possibly be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.

Recombinant Cucumis sativus Chromoplast-specific carotenoid-associated protein, chromoplast (CHRC)
MBS1304451-002mgYeast	MyBiosource	0.02mg(Yeast)	EUR 935

Recombinant Cucumis sativus Chromoplast-specific carotenoid-associated protein, chromoplast (CHRC)
MBS1304451-01mgEColi	MyBiosource	0.1mg(E-Coli)	EUR 935

Recombinant Cucumis sativus Chromoplast-specific carotenoid-associated protein, chromoplast (CHRC)
MBS1304451-01mgYeast	MyBiosource	0.1mg(Yeast)	EUR 1095

Catestatin (K388R,A395G,P403L) Variant / prepro-K388R,A395G,P403L Chromogranin A (Rat) - EIA Kit, extraction-free
EK-053-29	PHOENIX PEPTIDE	96 wells	EUR 603.72

RNA Extraction Kit
abx098089-100l	Abbexa	100 µl	EUR 812.5

RNA Extraction Kit
abx098089-1ml	Abbexa	1 ml	Ask for price

RNA Extraction Kit
abx098089-200l	Abbexa	200 µl	Ask for price

Gel Extraction Kit
MBS278007-200Preps	MyBiosource	200Preps	EUR 250

Gel Extraction Kit
MBS278007-50Preps	MyBiosource	50Preps	EUR 180

gPURE EXTRACTION KIT
IB47430	IBI Scientific	3ML	EUR 30.42
Description: (FOR CULTURED CELLS)

gPURE EXTRACTION KIT
IB47431	IBI Scientific	100ML	EUR 196.06
Description: (FOR CULTURED CELLS)

gPURE EXTRACTION KIT
IB47432	IBI Scientific	1000ML	EUR 1358.49
Description: (FOR CULTURED CELLS)

miRNA Extraction Kit
K1456-50	Biovision	50 Preps	EUR 661.2

ExoDNA? Extraction Kit
K1242-25	Biovision	each	EUR 705.6

ExoDNA? Extraction Kit
K1242-50	Biovision	each	EUR 1057.2

Nuclei Extraction Kit
MBS9718635-200Tests	MyBiosource	200Tests	EUR 230

Nuclei Extraction Kit
MBS9718635-50Tests	MyBiosource	50Tests	EUR 170

Nuclei Extraction Kit
MBS9718635-5x200Tests	MyBiosource	5x200Tests	EUR 1010

Plasmid Extraction Kit
MBS7608270-INQUIRE	MyBiosource	INQUIRE	Ask for price

Up RNA Extraction Kit
20-abx098087	Abbexa	Ask for price Ask for price	100 ml 3 ml

Up RNA Extraction Kit
abx098087-100l	Abbexa	100 µl	EUR 337.5

Up RNA Extraction Kit
abx098087-1ml	Abbexa	1 ml	Ask for price

Up RNA Extraction Kit
abx098087-200l	Abbexa	200 µl	Ask for price

Cysteine synthase, chloroplastic/chromoplastic Antibody
MBS7183330-INQUIRE	MyBiosource	INQUIRE	Ask for price

FFPE DNA Extraction Kit
EP016-50T	ELK Biotech	50T	EUR 140

FFPE DNA Extraction Kit
EP016	ELK Biotech	50T	EUR 140

Cell DNA Extraction Kit
TBS6007	Tribioscience	1000 samples	EUR 258

Mitochondrial Extraction Kit
MBS2571975-100Assays	MyBiosource	100Assays	EUR 260

Mitochondrial Extraction Kit
MBS2571975-50Assays	MyBiosource	50Assays	EUR 205

Mitochondrial Extraction Kit
MBS2571975-5x100Assays	MyBiosource	5x100Assays	EUR 1170

Quick Gel Extraction Kit
20-abx098085	Abbexa	Ask for price Ask for price	200 rxns 50 rxns

Plant RNA extraction Kit
20-abx098088	Abbexa	Ask for price Ask for price	100 ml 3 ml

Total RNA Extraction Kit
EP014-200T	ELK Biotech	200T	EUR 164