Tag: dh5alpha k12
Molecular Biology Methods in Streptomyces rimosus, a Producer of Oxytetracycline
Streptomyces rimosus is used for manufacturing of the broad-spectrum antibiotic oxytetracycline (OTC). S. rimosus belongs to Actinomyces species, a big group of microorganisms that produce various set of pure metabolites of excessive significance in lots of elements of our life. On this chapter, we describe particular molecular biology strategies and a classical homologous recombination strategy…
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Lyophilization and homogenization of biological samples improves reproducibility and reduces standard deviation in molecular biology techniques
Lyophilization is an economical technique for organic specimen preservation however detailed tissue-specific reference protocols are nonetheless missing. Furthermore, knowledge are restricted on the long-term stability of proteins and nucleic acids in lyophilized samples. Right here, we provide lyophilization protocols for varied rat and mouse tissues (kidney, coronary heart, liver, lung, aorta, and pores and skin)…
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