apexbio cell counting kit-8

Apexbio

Background

Cell proliferation will probably be detected by numerous methods. Cell proliferation induces an increase throughout the train of the mitochondrial dehydrogenases, which cleaves the tetrazolium salt to formazan.

The WST Cell Proliferation Colorimetric Assay Package deal plus provides one of the best and most delicate technique for quantification of cell proliferation and viability. Cell proliferation causes the rise throughout the amount of formazan dye normal that is straight proportional to the number of residing cells and will probably be quantified by measuring the absorbance of the dye decision at 440 nm using microtiter plate reader. The gear may be utilized for the analysis of cell proliferation in response to pharmaceutical compounds, cytotoxic compounds like anticancer drugs and many totally different toxic brokers. The assay may be used for the detection of cell proliferation in response to cytokines, mitogens, progress parts and nutritional vitamins, and so forth. The assay may very well be very easy and straightforward, merely add-and-read, requiring no solubilization, no washing and no harvesting steps, and is regular, sooner and additional delicate than MTS, MTT or XTT-based assays. Your whole assay will probably be carried out in a microtiter plate and correlates properly with the [3H]-thymidine incorporation assay.

Description

Our product Cell Counting Package deal-8 (CCK-8) current a additional helpful and delicate approach for the evaluation of cell amount dedication and cell proliferationtotoxicity assay than earlier strategies. The gear makes use of a extraordinarily water-soluble tetrazolium salt, WST-8, which produces a water-soluble formazan dye upon low cost throughout the presence of an electron mediator. The amount of the formazan generated by dehydrogenases is straight in proportion to the numbers of residing cells. The detection sensitivity by CCK-Eight is bigger than totally different tetrazolium salts resembling MTT, XTT, MTS or WST-1.

Apexbio Cell Counting Kit-8
Apexbio Cell Counting Kit-8

Choices

Additional Delicate than MTT, MTS, or WST-1

No Toxicity to Cell

Easier Steps, No pure solvents required

Regular One Bottle Reply, ready-to-use

 

Choices & Properties

Choices Fast and helpful Straightforward one-step course of; takes decrease than 4 hours. Makes use of a water-soluble tetrazolium salt WST reagent. The technique is really easy that requires no washing/harvesting/or solubilization steps and is faster and additional delicate than MTT/XTT/or MTS-based assays. Your whole assay will probably be carried out straight in a 96-well plate.
Supply Gel pack
Storage Circumstances Retailer at -20°C.

Cell Counting Kit-8 (CCK-8)

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Cell Counting Kit-8 (CCK-8)

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Cell Counting Kit (CCK)

20-abx098245
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  • 10 ml
  • 1 ml
  • 30 ml
  • 5 ml
  • Shipped within 5-10 working days.

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Cell Counting Kit-8 (CCK-8)

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Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

B34304 25 mL (2500 ractions)
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Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

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Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.

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