Methylated DNA Quantification Kit

methylflash methylated dna quantification kit

Product Choices
The MethylFlash™ Methylated DNA Quantification Bundle (Fluorometric) is a further refinement of our earlier SuperSense™ worldwide DNA methylation quantification kits by simplifying the workflow and enhancing the consistency of outcomes. It makes use of an progressive methodology to permit background alerts to be terribly low, eliminating the plate drying and blocking steps. Compared with chromatography-based methods equivalent to HPLC or mass spectrometry, this product gives an inexpensive answer to exactly measure ranges of 5-methylcytosine. The gear has the following advantages and choices:

Product Overview

The MethylFlash Methylated DNA Quantification Bundle (Fluorometric) is an entire set of optimized buffers and reagents to fluorometrically quantify worldwide DNA methylation by notably measuring ranges of 5-methylcytosine (5-mC) in a microplate-based format. Compared with LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash™ experience straight quantifies exact worldwide DNA methylation.

This gear may also be notably optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Bundle (Fluorometric) for concurrently quantifying every methylated DNA and hydroxymethylated DNA.

 

  • Fluorometric assay with easy-to-follow steps for consolation and velocity. All the method may be accomplished inside 4 hours.
  • Progressive gear composition permits background alerts to be terribly low, which eliminates the need for plate blocking and permits the assay to be straightforward, right, reliable, and fixed.
  • Extreme sensitivity, of which the detection prohibit may be as little as 50 pg of methylated DNA and accepts as little as 20 ng of enter genomic DNA
  • Optimized antibody and enhancer choices allow extreme specificity to 5-mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine all through the indicated focus fluctuate of the sample DNA.
  • Widespread optimistic and unfavourable controls are included, which might be applicable for quantifying methylated DNA from any species.
  • Strip-well microplate format makes the assay versatile: information or extreme throughput analysis.

Principle & Course of
The MethylFlash™ Methylated DNA Quantification Bundle (Fluorometric) contains all reagents wanted for the quantification of worldwide DNA methylation. On this assay, DNA is definite to strip wells which may be notably dealt with to have a extreme DNA affinity. The methylated fraction of DNA is detected using seize and detection antibodies after which quantified fluorometrically by finding out the absorbance in a fluorescence microplate spectrophotometer at excitation 530 and emission 590 nm. The amount of methylated DNA is proportional to the RFU (relative fluorescence fashions) measured, which may be calculated with the gear’s included formulation for relative methylation standing of two completely totally different DNA samples or absolute quantification of 5-methylcytosine (5-mC) using a typical curve.

Easy, Fast, and Versatile
All of the fluorometric assay has easy-to-follow steps for consolation and velocity, which may be achieved in 4 hours. The strip-well microplate format permits for a flexible assay in information or extreme throughput analysis. Widespread optimistic and unfavourable controls are included with the gear for quantifying methylated DNA from any species equivalent to mammals, vegetation, fungi, micro organism, and viruses.

Safe and Helpful
All the wished reagents, along with unfavourable controls and optimistic controls, for quantification of worldwide DNA methylation are conveniently packaged inside the gear. The direct fluorometric quantification of DNA samples replaces outdated or inferior methods and eliminates the need for DNA digestion/denaturation, radioactivity, extraction, or chromatography.

Methylated DNA Quantification Kit
Methylated DNA Quantification Equipment

Extraordinarily Delicate and Explicit
The novel course of and proprietary gear compositions allow for proper quantification of methylated DNA to be achieved with extreme sensitivity and specificity. The detection prohibit of the enter DNA may be as little as 50 pg of methylated DNA.

MethylFlash (Fluorometric) Competitor Z Competitor C
Assay Principle Direct ELISA Direct ELISA Indirect ELISA
Format 96-well plate 96-well plate 96-well plate
Sensitivity Superb detection prohibit: 0.05 ng of 5-mC DNA Good detection prohibit: >0.5 ng of 5-mC DNA Very poor detection prohibit: >1000 ng of 5-mC DNA
S/N Ratio >20 with very low background  <4 with extreme background  N/A*
Procedural Consolation No need for plate blocking and denaturation of DNA Requires plate blocking and DNA denaturation Requires plate blocking, DNA digestion, and antibody aggressive incubation in an additional plate
Protocol Time  <4 hr  <4 hr  >eight hr
DNA Form & Species Widespread for any species, every ssDNA and dsDNA Human and mouse ssDNA solely Quite a few species
Specificity Explicit to 5-mC solely Cross-reaction to 5-hmC N/A*
Quantitation Form Every relative and absolute quantification Relative Relative
Regular Administration Regular, quantitative in absolute amount of 5-mC, and may be universally used for any species Unstable, cannot be quantitative in amount, and will solely be used for human or mouse N/A*
Minimal Enter Amount 20 ng 100 ng 1000 ng
Accuracy of Detection Extreme, regular and quantified customary administration. Confirmed shut correlation with LC-MS analysis by clients Unclear, unstable and un-quantified customary administration N/A*
% 5-mC calculation Straightforward Tough Not Provided
Patented Method Certain No No
Status Very extreme printed citation rely Low printed citation rely Low printed citation rely
Assist Expertise Since 2006 Since 2013 Since 2011

 

Product Choices
The MethylFlash™ Methylated DNA Quantification Bundle (Colorimetric) is a further refinement of our earlier Methylamp™ worldwide DNA methylation quantification kits by simplifying the workflow and enhancing the consistency of outcomes. It makes use of an progressive methodology to permit background alerts to be terribly low, eliminating the plate drying and blocking steps. Compared with chromatography-based methods equivalent to HPLC or mass spectrometry, this product gives an inexpensive answer to exactly measure ranges of 5-methylcytosine. The gear has the following advantages and choices:

  • Colorimetric assay with easy-to-follow steps for consolation and velocity. All the method may be accomplished inside 4 hours.
  • Progressive gear composition permits background alerts to be terribly low, which eliminates the need for plate blocking and permits the assay to be straightforward, right, reliable, and fixed.
  • Extreme sensitivity, of which the detection prohibit may be as little as 0.2 ng of methylated DNA and accepts as little as 50 ng of enter genomic DNA.
  • Optimized antibody and enhancer choices allow extreme specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine all through the indicated focus fluctuate of the sample DNA.
  • Widespread optimistic and unfavourable controls are included, which might be applicable for quantifying methylated DNA from any species.
  • Strip-well microplate format makes the assay versatile: information or extreme throughput analysis.

Methylamp MS-qPCR Fast Kit 

P-1028
  • EUR 595.92
  • EUR 213.40
  • EUR 380.60
  • 200 Reactions
  • 100 Reactions
  • 200 Reactions

Fast Eva QPCR SuperMix Kit

K5052002 200 rxn
EUR 188

BrightGreen miRNA qPCR MasterMix-No Dye

MasterMix-mS 4 x 1.25 ml for 500 reactions
EUR 181.2

QuECHERS GC/MS Int Std

QUECR-MS-IS EACH
EUR 90.06

QuECHERS GC/MS QCSTD2

QUECR-MS-QC-2 EACH
EUR 189.24

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058200 200 reactions
EUR 253.2
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058400 400 reactions
EUR 384
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QuECHERS GC/MS QCSTD1 04

QUECR-MS-QC-1 EACH
EUR 182.4

QuECHERS GC/NPD and LC/MS

QUECR-NPD-MS-IS EACH
EUR 197.22

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 183.6

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 381.6
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 381.6
Description: Sealing Products; Sealing films - Axygen

Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXMT-KIT 30ML
EUR 237.12

Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXHM-KIT 30ML
EUR 245.1

2X Universal SYBR Green Fast qPCR Mix

RK21203 1 mL Ask for price

Genious 2X SYBR Green Fast qPCR Mix

RK21204 1 mL Ask for price

AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE

AGR-MS-1001 1/pk
EUR 2445.6
Description: Bioscience Mol Bio; Agarose

Methylamp DNA Modification Kit 

P-1001-1 40 Reactions
EUR 149.6

Methylamp DNA Modification Kit 

P-1001-2 80 Reactions
EUR 259.6

Methylamp DNA Modification Kit 

P-1001
  • EUR 416.70
  • EUR 149.60
  • EUR 259.60
  • 80 Reactions
  • 40 Reactions
  • 80 Reactions

Fast EvaGreen Master Mix for qPCR(200 rxn)

31003 2x1ML
EUR 244.8
Description: Minimum order quantity: 1 unit of 2x1ML

Fast EvaGreen Master Mix for qPCR (500 rxn)

31003-1 5x1ML
EUR 463.2
Description: Minimum order quantity: 1 unit of 5x1ML

Fast EvaGreen Master Mix for qPCR (5000 rxn)

31003-2 50x1ML
EUR 3739.2
Description: Minimum order quantity: 1 unit of 50x1ML

Fast Plus EvaGreen qPCR Master Mix (200 rxn)

31020 2x1mL
EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL