HIV-1 p24 Antigen ELISA 2.0

zeptometrix p24 extended range kit

Includes reagents that extend the standard curve up to 4ng/mL for either the HIV-1 p24 ELISA (Mfr. No. 0801111 or 0801200) or the HIV-1 p24 Antigen ELISA 2.0 (Mfr. No. 0801002 or 0801008)

 

Description

  • Allows samples containing p24 antigen up to 4ng/mL to be accurately quantified without extensive serial dilution

Specifications

Concentrated HIV-1 p24 Antigen Standard (0.3mL), SIGMAFAST™ OPD tablet sets (5 sets)
Store at 2° to 8°C
Sufficient for 5 x 96 determinations

PRINCIPLE OF THE TEST
Many specimens and tissue culture samples contain HIV-1 viral loads greater than the range of the standard curve of commercially available antigen ELISA’s. In order to quantify such samples, a series of expensive and time consuming dilutions must be made to adjust the sample concentration. The RETRO-TEK HIV-1 p24 Extended Range Kit includes reagents that extend the standard curve of the up to 4 ng/mL for either the HIV-1 p24 ELISA (catalog #: 0801111 or 0801200) or the HIV-1 p24 Antigen ELISA 2.0 (catalog #: 0801002 or 0801008). Therefore, samples containing p24 antigen up to 4 ng/mL may be accurately quantified without extensive serial dilution.

REAGENTS


Materials Supplied

• Concentrated HIV-1 p24 Antigen Standard (0.3mL): Contains detergent disrupted, heat inactivated viral antigen at a concentration of 80 ng/mL, added protein, detergent and 2-chloroacetamide.
• Substrate (5 sets): SIGMAFASTTM OPD tablet sets.
Contains: 5 OPD tablets (o-phenylenediamine dihydrochloride) [silver foil]
Contains: 5 urea-hydrogen peroxide tablets [gold foil]

SIGMAFASTTM is a registered trademark of the Sigma-Aldrich Company.

Storage: Store all kit reagents at 2°-8°C. Materials Required but Not Supplied

• HIV-1 p24 Antigen ELISA (catalog #: 0801111 or 0801200) or
HIV-1 p24 Antigen ELISA 2.0 (catalog #: 0801002 or 0801008)
• Distilled or deionized water
• Test tubes and racks for preparing specimen and standard dilutions
• Adjustable micropipettes, single and multichannel
• Incubator capable of maintaining 37º± 1º C
• Timer
• Graduated cylinders and assorted beakers
• Automatic microplate washer or manual vacuum aspiration equipment
• 1% sodium hypochlorite as disinfectant. May be prepared from household bleach

 

zeptometrix p24 extended range kitPRECAUTIONS

• OPD is a possible carcinogen. Avoid contact. Use gloves when handling tablets.
• Use Universal Precautions when handling test specimens and HIV-1 p24 Antigen Standard.*
• Disposal: When examining human source material or other potentially infectious specimens, adhere to all applicable local, state and federal regulations regarding disposal of hazardous materials.
• To avoid cross-contamination, use separate pipet tips for each specimen.
* from MMWR, June 24,1988, Vol. 37, No.24, pp. 377-382, 387-8

zeptometrix p24 extended range kit
zeptometrix p24 extended range kit

TEST PROCEDURE
Allow all reagents to reach room temperature before use. Proceed with the RETRO-TEK HIV-1 p24 ELISA according to the product insert with the following changes:

1. Replace the HIV-1 p24 Antigen Standard supplied with the p24 ELISA kit with the HIV-1 p24 Antigen Extended Range Standard.
2. Substitute the TMB Substrate supplied with the p24 ELISA kit with the OPD Substrate Solution and incubate microplate for 30 minutes at room temperature.
Please Note: Due to plate reader differences, the high standard absorbance values may be out of range. Read plate at 435nm or 405nm if necessary.

CALCULATION AND INTERPRETATION OF RESULTS
Calculate the mean absorbance for each p24 standard and test sample. Plot the concentration of HIV-1 p24 Antigen Standard (pg/mL) on the X-axis versus the mean optical density (OD) for each standard on the Y-axis. The HIV-1 p24 concentration of unknown samples is best determined by using a 4-parameter logistic regression. Alternatively, a point-to-point algorithm may be used. Only readings which fall within the range of the standard curve may be quantified. Be sure to correct for all dilutions, including the 1.1 dilution made during the addition of Lysing Buffer.
Below is an example of a typical standard curve for the RETRO-TEK HIV-1 p24 Antigen ELISA (ZEPTOMETRIX catalog # 0801111 or 0801200).

SmartCheck™ Tri-color Extended Range Protein Marker, 12 bands 9-245 kDA, 2x250ul

PR5012-TC 1 each
EUR 170.47

SmartCheck™ Tri-color Extended Range Plus Protein Marker, 13 bands 9-245 kDA, 2x250ul

PR5013-TC 1 each
EUR 183.55

EMPTY RACK FOR AXYGEN MULTIRACK 200UL EXTENDED LENGTH, 300UL EXTENDED LENTGH, AND 1000 UL EXTENDED LENGTH

MR-R-B 5/pk
EUR 112.8
Description: Non Filter Tips; Multi Rack Tips

Extended Warranty 50

EW-50 1each
EUR 1114.8
Description: Extended warranty on a BB50-DX Bullet Blender, from 2 to 4 years. Includes priority on repairs, parts, labor and domestic shipping.

Extended Warranty BBX

EW-BBX 1each
EUR 770.4
Description: Extended warranty on a BBX series Bullet Blender, from 2 to 4 years. Includes priority on repairs, parts, labor and domestic shipping.

Extended Warranty BBY

EW-BBY 1each
EUR 830.4
Description: Extended warranty on a BBY series Bullet Blender, from 2 to 4 years. Includes priority on repairs, parts, labor and domestic shipping.

Extended Warranty GOLD

EW-GOLD 1each
EUR 1387.2
Description: Extended warranty on a GOLD series Bullet Blender, from 2 to 4 years. Includes priority on repairs, parts, labor and domestic shipping.

Extended Warranty BlotBot

EW-FRB 1each
EUR 1437.6
Description: Extended warranty on a Freedom Rocker BlotBot, from 2 to 4 years. Includes priority on repairs, parts, labor and domestic shipping.

Linearity FD BNP (Extended)

K737M-5 5 x 1 mL
EUR 374

Finntip 1000 Extended, Bag (1000)

TH9401410 each
EUR 171.13

Finntip 200 Extended, Box (400)

TH9400100 each
EUR 81.75

Prestige Autoclave 2100 Extended

AUT4502 EACH
EUR 1504.8

Axygen Empty rack for MultiRack 200uL extended length 300uL and 1000 uL extended length - PK5

PIP1328 PK5
EUR 137.7

Finntip 1000 Extended, Rack (5x96)

TH9401420 each
EUR 96.47

Finntip 200 Extended, Rack (10x96)

TH9400130 each
EUR 231.08

PepPool: CEF extended (CD8), human

3618-1 One vial with 25 ug of each peptide
EUR 162.75

Rat Extended Synaptotagmin 1 (ESYT1) ELISA Kit

abx391308-96tests 96 tests
EUR 1093.2

Rat Extended Synaptotagmin 1 (ESYT1) ELISA Kit

abx391308-100l 100 µl Ask for price

Rat Extended Synaptotagmin 1 (ESYT1) ELISA Kit

abx391308-50l 50 µl
EUR 687.5

ClipTip 12.5µl Tips Extended (10x96)

TH94410060 each
EUR 70.31

Prestige Autoclave 2100 Extended+ - EACH

AUT4504 EACH
EUR 2739.31

Mouse Extended Synaptotagmin 2 (ESYT2) ELISA Kit

abx389231-96tests 96 tests
EUR 1093.2

Mouse Extended Synaptotagmin 1 (ESYT1) ELISA Kit

abx389232-96tests 96 tests
EUR 1093.2

Mouse Extended Synaptotagmin 3 (ESYT3) ELISA Kit

abx389233-96tests 96 tests
EUR 1093.2

Human Extended Synaptotagmin 2 (ESYT2) ELISA Kit

abx384873-96tests 96 tests
EUR 1093.2

Human Extended Synaptotagmin 3 (ESYT3) ELISA Kit

abx384874-96tests 96 tests
EUR 1093.2

Human Extended synaptotagmin- 2, ESYT2 ELISA KIT

ELI-26958h 96tests
EUR 696

Mouse Extended synaptotagmin- 1, Esyt1 ELISA KIT

ELI-48047m 96tests
EUR 736