Epigentek Chromoplasts Chromatin immunoprecipitaton Extraction Kit
Background Information
Chromatin immunoprecipitaton (ChIP) provides an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions could possibly be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could possibly be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.
Product Overview
The ChromaFlash Chromatin Extraction Package deal is an entire set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle may be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.
- Terribly fast course of: your whole course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
- Helpful and versatile: the bundle is suitable for getting ready every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
- Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.
Principle & Course of
The ChromaFlash™ Chromatin Extraction Package deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.
Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, modern and frozen tissues, and so forth. The amount of cells and tissues for each preparation could possibly be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues. Yield of chromatin is roughly 4 ug per 106 cells or per 50 mg tissues.
Background Information
Chromatin immunoprecipitation (ChIP) provides an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions could possibly be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could possibly be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.
RNA Extraction Kit |
|||
abx098089-1ml | Abbexa | 1 ml | Ask for price |
RNA Extraction Kit |
|||
abx098089-200l | Abbexa | 200 µl | Ask for price |
gPURE EXTRACTION KIT |
|||
IB47430 | IBI Scientific | 3ML | EUR 30.42 |
Description: (FOR CULTURED CELLS) |
gPURE EXTRACTION KIT |
|||
IB47431 | IBI Scientific | 100ML | EUR 196.06 |
Description: (FOR CULTURED CELLS) |
gPURE EXTRACTION KIT |
|||
IB47432 | IBI Scientific | 1000ML | EUR 1358.49 |
Description: (FOR CULTURED CELLS) |
miRNA Extraction Kit |
|||
K1456-50 | Biovision | 50 Preps | EUR 661.2 |
ExoDNA? Extraction Kit |
|||
K1242-25 | Biovision | each | EUR 705.6 |
ExoDNA? Extraction Kit |
|||
K1242-50 | Biovision | each | EUR 1057.2 |
Up RNA Extraction Kit |
|||
20-abx098087 | Abbexa |
|
|
Up RNA Extraction Kit |
|||
abx098087-100l | Abbexa | 100 µl | EUR 337.5 |
Up RNA Extraction Kit |
|||
abx098087-1ml | Abbexa | 1 ml | Ask for price |
Up RNA Extraction Kit |
|||
abx098087-200l | Abbexa | 200 µl | Ask for price |
FFPE DNA Extraction Kit |
|||
EP016 | ELK Biotech | 50T | EUR 140 |
Cell DNA Extraction Kit |
|||
TBS6007 | Tribioscience | 1000 samples | EUR 258 |
Total RNA Extraction Kit |
|||
EP014 | ELK Biotech | 50T | EUR 164 |
Quick Gel Extraction Kit |
|||
20-abx098085 | Abbexa |
|
|
Plant RNA extraction Kit |
|||
20-abx098088 | Abbexa |
|
|
Blood DNA Extraction Kit |
|||
TBS6004 | Tribioscience | 1000 samples | EUR 298 |
Urine DNA Extraction Kit |
|||
UR004 | Geneaid Biotech | each | EUR 3 |
Urine DNA Extraction Kit |
|||
UR050 | Geneaid Biotech | each | EUR 65 |
Urine DNA Extraction Kit |
|||
UR100 | Geneaid Biotech | each | EUR 117 |
Quick Gel Extraction Kit |
|||
abx098085-100l | Abbexa | 100 µl | EUR 250 |
Quick Gel Extraction Kit |
|||
abx098085-1ml | Abbexa | 1 ml | Ask for price |
Quick Gel Extraction Kit |
|||
abx098085-200l | Abbexa | 200 µl | EUR 350 |
Plant RNA extraction Kit |
|||
abx098088-100l | Abbexa | 100 µl | EUR 337.5 |
Plant RNA extraction Kit |
|||
abx098088-1ml | Abbexa | 1 ml | Ask for price |
Plant RNA extraction Kit |
|||
abx098088-200l | Abbexa | 200 µl | Ask for price |
Gel Extraction Kit (100 preps) |
|||
9K-006-0001 | Bio Basic | 100preps | EUR 283.42 |
Gel Extraction Kit (200 preps) |
|||
9K-006-0002 | Bio Basic | 200preps, 200prep | EUR 418.09 |
FFPE Protein Extraction Kit |
|||
BSP058 | Bio Basic | 50Preps | EUR 122.64 |
Plant Tissue Extraction Kit |
|||
K296-50 | Biovision | each | EUR 548.4 |
Exosomal RNA Extraction Kit |
|||
P200R | 101Bio | 20 rxn | EUR 389 |
Exosomal DNA Extraction Kit |
|||
P230-25 | 101Bio | 25 rxn | EUR 339 |