ExoQuick method for Exosome Isolation kit

exosome isolation kit

General description

Effective isolation of exosomes from plasma

“Therefore, we looked at the ExoQuick method for further studies, as these samples required much less sample input, an important advantage when working with clinical samples and mouse models.” By isolating exosomes from plasma, fibrinogen and fibrin can prevent effective recovery. By pre-treating plasma with thrombin, fibrinogen can be converted to fibrin and easily pre-clarified by a single centrifugation step. The resulting serum-like solution can now be treated with ExoQuick, the original formulation, for quantitative isolation of exosomes compatible with high flow methods.

 

ExoQuick fast, non-ultracentrifugation method:

  • Save time and effort
  • It is easily scalable
  • Save a valuable sample
  • It provides high yields of high quality and functional exosomes.
  • It can be used to isolate exosomes for a wide range of downstream applications, including
  • Biomarker studies
  • Exosomal miRNA profiles
  • Exosomal proteomics
  • Lipidomics / exosomal metabolomics
  • Functional studies, such as cell-to-cell signaling.
  • Basic biology, such as its role in tumorigenesis.
exosome isolation kit
exosome isolation kit

ExoQuick is a proprietary polymer that gently precipitates exosomes. For isolation of exosomes from plasma, you can first defibrinize your sample with thrombin and then proceed with the standard ExoQuick protocol for pre-purifying your sample of cells and cell debris, adding the appropriate amount of ExoQuick, cooling, and then centrifuging (see product manual for protocol information). Your exosomes will be in the pellet, ready for resuspension in a suitable solution.

In electron microscopy studies, exosomes isolated with ExoQuick look like exosomes isolated by ultracentrifugation 1-2, and these exosomes are also active in numerous functional assays1-3.

Exosomes isolated with ExoQuick can be used for all types of protein profiling and characterization studies, such as mass spectrometry, Western blotting, ELISA and more. Higher protein yield is obtained by ExoQuick purification than by chromatography, DynaBeads or ultracentrifugation.

Exosomes isolated with ExoQuick also provide excellent samples for the study of exosome-associated nucleic acids, such as microRNAs, siRNAs, and even mRNAs. Quantitative analysis techniques such as qPCR, microarray studies, and next-generation sequencing are compatible with nucleic acids isolated from exosomes purified with ExoQuick.

Supported by an increasing number of publications, ExoQuick is often the best choice for researchers working with low sample volumes, such as clinical research samples or small animal models.

 

CD63 Capture Exosome Isolation Kit

E601E005 20 tests
EUR 2500

CD81 Capture Exosome Isolation Kit

E601E006 20 tests
EUR 2500

Breast cancer Exosome Isolation Kit

E601E002 20 tests
EUR 2500

EPCAM Capture Exosome Isolation Kit

E601E003 20 tests
EUR 2500

HSP70 Capture Exosome Isolation Kit

E601E007 20 tests
EUR 2500

Exosome Isolation kit (for serum & plasma)

P101 - Ask for price

Exosome Isolation kit (for serum & plasma)

P101L - Ask for price

Exosome Isolation kit (for serum & plasma)

P101S - Ask for price

Custom cell exosome isolation

S101 -
EUR 700

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

Disease cell exosome isolation

S102 - Ask for price

Exosome Isolation kit (forHuman Body Fluid)

P121 - Ask for price

Exosome Isolation kit (forHuman Body Fluid)

P121L - Ask for price

Exosome Isolation kit (forHuman Body Fluid)

P121S - Ask for price

Exosome Isolation kit (for cell culture media)

P100 - Ask for price

Exosome Isolation kit (for cell culture media)

P100L - Ask for price

Exosome Isolation kit (for cell culture media)

P100S - Ask for price

Easy-Exo Saliva Exosome Isolation Kit, 95% pure

P530 - Ask for price

Easy-Exo Saliva Exosome Isolation Kit, 95% pure

P530S - Ask for price

Exosome Isolation kit (for stem cell culture media)

P107S - Ask for price

Exosome Isolation kit (for stem cell culture media)

P107 - Ask for price

VEX Exosome Isolation Reagent (from serum)

R602 10 ml
EUR 769.2

VEX Exosome Isolation Reagent (from plasma)

R603 10 ml
EUR 769.2

ExoPure? Reagent (Overall Exosome Isolation, urine)

M1003-25 each
EUR 992.4

ExoPure? Reagent (Overall Exosome Isolation, urine)

M1003-50 each
EUR 1083.6

ExoPure? Reagent (Overall Exosome Isolation, cell media)

M1002-25 each
EUR 992.4

ExoPure? Reagent (Overall Exosome Isolation, cell media)

M1002-50 each
EUR 1083.6

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

VEX Exosome Isolation Reagent (from cell culture media)

R601 50 ml
EUR 706.8

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ - Ask for price