ExoQuick method for Exosome Isolation kit
General description
Effective isolation of exosomes from plasma
“Therefore, we looked at the ExoQuick method for further studies, as these samples required much less sample input, an important advantage when working with clinical samples and mouse models.” By isolating exosomes from plasma, fibrinogen and fibrin can prevent effective recovery. By pre-treating plasma with thrombin, fibrinogen can be converted to fibrin and easily pre-clarified by a single centrifugation step. The resulting serum-like solution can now be treated with ExoQuick, the original formulation, for quantitative isolation of exosomes compatible with high flow methods.
ExoQuick fast, non-ultracentrifugation method:
- Save time and effort
- It is easily scalable
- Save a valuable sample
- It provides high yields of high quality and functional exosomes.
- It can be used to isolate exosomes for a wide range of downstream applications, including
- Biomarker studies
- Exosomal miRNA profiles
- Exosomal proteomics
- Lipidomics / exosomal metabolomics
- Functional studies, such as cell-to-cell signaling.
- Basic biology, such as its role in tumorigenesis.
ExoQuick is a proprietary polymer that gently precipitates exosomes. For isolation of exosomes from plasma, you can first defibrinize your sample with thrombin and then proceed with the standard ExoQuick protocol for pre-purifying your sample of cells and cell debris, adding the appropriate amount of ExoQuick, cooling, and then centrifuging (see product manual for protocol information). Your exosomes will be in the pellet, ready for resuspension in a suitable solution.
In electron microscopy studies, exosomes isolated with ExoQuick look like exosomes isolated by ultracentrifugation 1-2, and these exosomes are also active in numerous functional assays1-3.
Exosomes isolated with ExoQuick can be used for all types of protein profiling and characterization studies, such as mass spectrometry, Western blotting, ELISA and more. Higher protein yield is obtained by ExoQuick purification than by chromatography, DynaBeads or ultracentrifugation.
Exosomes isolated with ExoQuick also provide excellent samples for the study of exosome-associated nucleic acids, such as microRNAs, siRNAs, and even mRNAs. Quantitative analysis techniques such as qPCR, microarray studies, and next-generation sequencing are compatible with nucleic acids isolated from exosomes purified with ExoQuick.
Supported by an increasing number of publications, ExoQuick is often the best choice for researchers working with low sample volumes, such as clinical research samples or small animal models.
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| E601E005 | EnoGene | 20 tests | EUR 2500 |
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| E601E007 | EnoGene | 20 tests | EUR 2500 |
) ExoQualiTM Exosome Isolation Kit (Urine) |
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| CEIK-003L-10T | Creative Bioarray | 10 T | Ask for price |
ExoQualiTM Exosome Isolation Kit (Urine) |
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| CEIK-003L-30T | Creative Bioarray | 30 T | Ask for price |
) ExoQualiTM Exosome Isolation Kit (Serum) |
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| CEIK-005L-10T | Creative Bioarray | 10 T | Ask for price |
ExoQualiTM Exosome Isolation Kit (Serum) |
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| CEIK-005L-30T | Creative Bioarray | 30 T | Ask for price |
) ExoQualiTM Exosome Isolation Kit (Plasma) |
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| CEIK-004L-10T | Creative Bioarray | 10 T | Ask for price |
ExoQualiTM Exosome Isolation Kit (Plasma) |
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| CEIK-004L-30T | Creative Bioarray | 30 T | Ask for price |
) Exosome Isolation kit (for serum & plasma) |
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| P101 | 101Bio | 10 rxn | EUR 199 |
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| P101L | 101Bio | 40 rxn | EUR 769 |
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| P101S | 101Bio | 2 rxn | EUR 69 |
) Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS) |
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| P101NGS | 101Bio | - | Ask for price |
) Exosome Isolation kit (forHuman Body Fluid) |
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| P121 | 101Bio | 10 rxn | EUR 199 |
Exosome Isolation kit (forHuman Body Fluid) |
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| P121L | 101Bio | 40 rxn | EUR 769 |
Exosome Isolation kit (forHuman Body Fluid) |
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| P121S | 101Bio | 2 rxn | EUR 69 |
) ExoQualiTM Exosome Isolation Kit (Cell Media) |
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| CEIK-001L-10T | Creative Bioarray | 10 T | Ask for price |
ExoQualiTM Exosome Isolation Kit (Cell Media) |
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| CEIK-001L-30T | Creative Bioarray | 30 T | Ask for price |
) ExoQualiTM Exosome Isolation Kit (Plasma & Serum) |
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| CEIK-006L-10T | Creative Bioarray | 10 T | Ask for price |
ExoQualiTM Exosome Isolation Kit (Plasma & Serum) |
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| CEIK-006L-30T | Creative Bioarray | 30 T | Ask for price |
) ExoQualiTM Exosome Isolation Kit (All in one) |
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| CEIK-008L-10T | Creative Bioarray | 10 T | Ask for price |
ExoQualiTM Exosome Isolation Kit (All in one) |
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| CEIK-008L-30T | Creative Bioarray | 30 T | Ask for price |
) Exosome Isolation kit (for cell culture media) |
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| P100 | 101Bio | 10 rxn | EUR 199 |
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| P100L | 101Bio | 40 rxn | EUR 769 |
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| P100S | 101Bio | 2 rxn | EUR 69 |
) ExoQualiTM Exosome Isolation Kit (Stem Cell Media) |
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| CEIK-002L-10T | Creative Bioarray | 10 T | Ask for price |
ExoQualiTM Exosome Isolation Kit (Stem Cell Media) |
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| CEIK-002L-30T | Creative Bioarray | 30 T | Ask for price |
) ExoQualiTM Exosome Isolation Kit (other body fluids) |
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| CEIK-007L-10T | Creative Bioarray | 10 T | Ask for price |