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β-Defensins from common goby (Pomatoschistus microps) and silver trevally (Pseudocaranx georgianus): Molecular characterization and phylogenetic analysis

Antimicrobial peptides (AMPs) are biologically active molecules involved in host defense present in a variety of organisms. They are an integral component of innate immunity, forming a front line of defense against potential pathogens, including antibiotic-resistant ones. Fishes are proven to be a prospective source of AMPs as they are constantly being challenged by a…

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MOLECULAR INVESTIGATION AND PHYLOGENETIC ANALYSIS OF ANAPLASMOSIS IN DOGS

  Anaplasmosis is a widespread vector-borne illness affecting canines, and Anaplasma platys is the key etiological agent of the illness. The research examines anaplasmosis molecular prevalence, associated threat elements, and alteration of hematological variables in Anaplasma-affected canines. A complete of 150 blood samples have been collected from canines within the district of Lahore, Pakistan. The…

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First report of molecular and phylogenetic evaluation of Physaloptera praeputialis in naturally contaminated stray cats from India   Nematodes of the genus Physaloptera are globally distributed and infect a large number of hosts. Their life cycle includes orthopterans and coleopterans as intermediate hosts. The morphological characters alone are insufficient to detect and differentiate Physaloptera spp. from its congeners. Furthermore,…

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Phylogenetic study of Theileria ovis and Theileria lestoquardi in sheep from Egypt: Molecular evidence and genetic characterization

Background and intention: Ovine theileriosis brought on by Theileria ovis and Theileria lestoquardi is a crucial infectious illness affecting small ruminants in areas of the tropic and subtropic zones. There may be restricted research about ovine theileriosis in Egypt; so the current research goals to evaluate the incidence of ovine theileriosis in Egypt on the molecular stage. <sturdy class=”sub-title”> Supplies…

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An overview of molecular biology and nanotechnology based analytical methods for the detection of SARS-CoV-2: promising biotools for the rapid diagnosis of COVID-19

At present, the 2019 novel coronavirus (2019-nCoV) is drastically affecting 214 nations, inflicting extreme pneumonia in sufferers, which has resulted in lockdown being applied in a number of nations to cease its native transmission. Contemplating this, the fast screening and correct detection of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2; 2019-nCoV) play a vital position…

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Molecular Biology Methods in Streptomyces rimosus, a Producer of Oxytetracycline

Streptomyces rimosus is used for manufacturing of the broad-spectrum antibiotic oxytetracycline (OTC). S. rimosus belongs to Actinomyces species, a big group of microorganisms that produce various set of pure metabolites of excessive significance in lots of elements of our life. On this chapter, we describe particular molecular biology strategies and a classical homologous recombination strategy…

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Lyophilization and homogenization of biological samples improves reproducibility and reduces standard deviation in molecular biology techniques

Lyophilization is an economical technique for organic specimen preservation however detailed tissue-specific reference protocols are nonetheless missing. Furthermore, knowledge are restricted on the long-term stability of proteins and nucleic acids in lyophilized samples. Right here, we provide lyophilization protocols for varied rat and mouse tissues (kidney, coronary heart, liver, lung, aorta, and pores and skin)…

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Entosis: From Cell Biology to Clinical Cancer Pathology

Entosis is a phenomenon, wherein one cell enters a second one. New clinico-histopathological research of entosis prompted us to summarize its significance in most cancers. It seems that entosis may be a novel, impartial prognostic predictor consider most cancers histopathology. We briefly focus on the organic foundation of entosis, adopted by a abstract of printed…

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Molecular Biology of Basal and Squamous Cell Carcinomas

The prevalent keratinocyte-derived neoplasms of the pores and skin are basal cell carcinoma and squamous cell carcinoma. Each so-called non-melanoma pores and skin cancers comprise the most typical cancers in people by far. Widespread danger components for each tumor entities embrace solar publicity, DNA restore deficiencies resulting in chromosomal instability, or immunosuppression. But, basic variations…

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Propofol Affects Non-Small-Cell Lung Cancer Cell Biology By Regulating the miR-21/PTEN/AKT Pathway In Vitro and In Vivo

Background: Propofol is a typical sedative-hypnotic drug historically used for inducing and sustaining basic anesthesia. Current research have drawn consideration to the nonanesthetic results of propofol, however the potential mechanism by which propofol suppresses non-small-cell lung most cancers (NSCLC) development has not been totally elucidated. Strategies: For the in vitro experiments, we used propofol (0, 2, 5,…

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