Exosome Capture And Qualification Assay Kit

qualification assay kit

Exo-TEST: ready to use ELISA quantification kits

 

ExoTEST is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions.

 

ExoTEST consists of ELISA plates pre-coated with patented panexosome antibodies that allow specific capture of exosomes from a variety of biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is then performed using appropriate detection antibodies against exosome-associated antigens, which can be either generic or cell / tissue specific exosomes. Lyophilized exosome standards characterized by protein content and particle number (NTA) allow the quantification of an unknown sample by a standard calibration curve.

Applications: Capture and quantification of exosomes from human biofluids and culture media. Exosome complex profiling. Preclinical research on non-invasive biomarkers for the detection and monitoring of a number of pathological conditions (inflammation, cancer, neurodegeneration, etc.).

Advantages: Ready to use, no initial purification of exosomes is required. User-friendly and suitable for the analysis of multiple markers. Available in TEST format (limited to 3 ELISA strips, 24 wells).

Reference:

Musante, L., Tataruch-Weinert, D., Kerjaschki, D., Henry, M., Meleady, P., & Holthofer, H. (2017). Residual extracellular urinary vesicles in ultracentrifugation supernatants after enrichment by dialysis by hydrostatic filtration. Journal of Extracellular Vesicles, 6 (1), 1267896.

Foster, B. P., Balassa, T., Benen, T. D., Dominovic, M., Elmadjian, G. K., Florova, V., … & Kyvelidou, C. (2016). Extracellular vesicles in the blood, milk and body fluids of the female and male urogenital tracts and with special regard to reproduction. Critical Reviews in Clinical Laboratory Sciences, (just accepted), 1-47.

Tas, F., Karabulut, S., Tilgen Yasasever, C., & Duranyildiz, D. (2015). Clinical significance of serum caveolin -1 levels in patients with melanoma. International Journal of Dermatology.

Zarovni, N., Corrado, A., Guazzi, P., Zocco, D., Lari, E., Radano, G., … & Chiesi, A. (2015). Integrated isolation and quantitative analysis of exosome-transmitted proteins and nucleic acids using immunocapsura approaches.

 

qualification assay kit
qualification assay kit

Methods.

Miller, I. V., & Grunewald, T. G. (2015). Tumor-derived exosomes: Tiny envelopes for big stories. Biology of the Cell, 107 (9), 287-305.

Hong, C. S., Muller, L., Boyiadzis, M., & Whiteside, T. L. (2014). Isolation and characterization of CD34 + blast-derived exosomes in acute myeloid leukemia. PLoS One, 9 (8), e103310.

Tetta, C., Ghigo, E., Silengo, L., Deregibus, M. C., & Camussi, G. (2013). Extracellular vesicles as an emerging mechanism of communication between cells. Endocrine, 44 (1), 11-19.

Vishnubhatla, I., Corteling, R., Stevanato, L., Hicks, C., & Sinden, J. (2014). Development of stem cell-derived exosomes as cell-free regenerative medicine. J. Circ. Biomark, 143, 2.

Logozzi, M., De Milito, A., Lugini, L., Borghi, M., Calabrò, L., Spada, M., … & Fais, S. (2009). High levels of exosomes expressing CD63 and caveolin-1 in the plasma of patients with melanoma. (4), e5219.

 

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132
  • Category: Cas9

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805
  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Glycosaminoglycans Assay Kit

6022 1 kit
EUR 419.75
Description: Glycosaminoglycans Assay Kit

DNA Assay Kit

6023 1 kit
EUR 180.5
Description: DNA Assay Kit

Hemoglobin Assay Kit

6024 1 kit
EUR 180.5
Description: Hemoglobin Assay Kit

Proteasome Assay Kit

55R-1341 100 assays
EUR 654
Description: Assay Kit for detection of Proteasome in the research laboratory

Calpain Assay Kit

55R-1342 100 assays
EUR 779
Description: Assay Kit for detection of Calpain in the research laboratory

Glutathione Assay Kit

55R-1343 100 assays
EUR 654
Description: Assay Kit for detection of Glutathione in the research laboratory

Glutathione Assay Kit

55R-1354 100 assays
EUR 895
Description: Assay Kit for detection of Glutathione activity in the research laboratory

HDAC Assay Kit

55R-1371 100 assays
EUR 673
Description: Assay Kit for detection of HDAC in the research laboratory

HDAC Assay Kit

55R-1372 100 assays
EUR 673
Description: Assay Kit for detection of HDAC in the research laboratory

HAT Assay Kit

55R-1373 100 assays
EUR 740
Description: Assay Kit for detection of HAT in the research laboratory

SOD Assay Kit

55R-1374 100 assays
EUR 602
Description: Assay Kit for detection of SOD in the research laboratory

HDAC3 Assay Kit

55R-1377 100 assays
EUR 693
Description: Assay Kit for detection of HDAC3 in the research laboratory

HDAC8 Assay Kit

55R-1379 100 assays
EUR 689
Description: Assay Kit for detection of HDAC8 in the research laboratory

ATP Assay Kit

55R-1380 100 assays
EUR 809
Description: Assay Kit for detection of ATP in the research laboratory

ADP Assay Kit

55R-1381 100 assays
EUR 809
Description: Assay Kit for detection of ADP in the research laboratory

FAD Assay Kit

55R-1382 100 assays
EUR 732
Description: Assay Kit for detection of FAD in the research laboratory

PEP Assay Kit

55R-1384 100 assays
EUR 876
Description: Assay Kit for detection of PEP in the research laboratory

Ammonia Assay Kit

55R-1388 100 assays
EUR 740
Description: Assay Kit for detection of Ammonia in the research laboratory

cAMP Assay Kit

55R-1389 100 assays
EUR 706
Description: Assay Kit for detection of cAMP activity in the research laboratory

cGMP Assay Kit

55R-1390 100 assays
EUR 706
Description: Assay Kit for detection of cGMP activity in the research laboratory

Urea Assay Kit

55R-1391 100 assays
EUR 740
Description: Assay Kit for detection of Urea in the research laboratory

Calcium Assay Kit

55R-1392 250 assays
EUR 586
Description: Assay Kit for detection of Calcium activity in the research laboratory

Magnesium Assay Kit

55R-1393 100 assays
EUR 654
Description: Assay Kit for detection of Magnesium in the research laboratory