Cusao Proteinase K

Proteinase k, recombinant

Proteinase Okay (Lyophilized)

For Basic Digestion of Protein in Pure Samples

  • Lively over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
  • Purified to take away RNase and DNase actions

Secure at Room Temperature and Straightforward to Use

  • Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% prepare at temperatures as rather a lot as 60°C
  • No resuspension or thawing ahead of use
  • Cat.# MC5005, MC5008 provided at a highlight of 20mg/ml

 

Proteinase K, recombinant
Proteinase K, recombinant



Highlights

• Prepared-to-use reply
• Lively in a variety of response circumstances

Capabilities

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

 

Proteinase Okay – one in every of many sometimes used enzymes in molecular biology.

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.



Keep in mind

• The really useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The prepare of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Okay in route of autolysis, will enhance the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Okay
• Secure over an enormous pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum prepare at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.

 

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular prepare.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the best high quality product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.

Decisions

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Excessive prepare and distinctive purity.
  • Lively at excessive temperatures (as rather a lot as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in quite a lot of options.
  • Secure over an enormous pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Capabilities:

  • Extraction of DNA and RNA from fully completely totally different beginning offers.
  • Purification of goal provides from contaminating proteins.
  • Elimination of DNases and RNases all by nucleic acids isolation.

 

Proteinase K
P2180-100 1g
EUR 627.6
Proteinase K
GE0990-100MG 100 mg
EUR 112.8
Proteinase K
GE0990-1G 1 g
EUR 495.6
Proteinase K
GE0990-250MG 250 mg
EUR 184.8
Proteinase K
GE0990-25MG 25 mg
EUR 69.6
Proteinase K
GE0990-500MG 500 mg
EUR 298.8
Proteinase K
G029 1.0 ml
EUR 170.4
Proteinase K
K1037-1 1 ml
EUR 80.4
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.
Proteinase K
K1037-5 5 ml
EUR 129.6
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.
Proteinase K
Z5050002 100 mg
EUR 123
Proteinase K
DE102-01 1 ml
EUR 11.6
Proteinase K
GE0990-1 1
EUR 379.6
Proteinase K
GE0990-100 100
EUR 63.3
Proteinase K
GE0990-25 25
EUR 27.8
Proteinase K
GE0990-250 250
EUR 123.4
Proteinase K
GE0990-500 500
EUR 217.6
Proteinase K - 1g
PB0451 1g
EUR 667.61
10x Proteinase K
TG4039 0.5ml
EUR 160.8
Proteinase K protein
30R-2901 500 mg
EUR 510
Description: Purified recombinant Human Proteinase K protein
Proteinase K 100 mg
DD91553 EACH
EUR 201.6
Proteinase K Solution
BIO-37084 5ml @ 20mg/ml Ask for price
Proteinase K Solution
BIO-37085 5 x 5ml @ 20mg/ml Ask for price
Proteinase K Antibody
6210-100 each
EUR 352.8
Proteinase K Antibody
6210-30T each
EUR 175.2
PROTEINASE K SOLUTION
501-PK 5 ml
EUR 182.4
Description: Genomic PCR Grade Proteinase K Solution
Proteinase K solution
GZ5904-100ML 100 ml
EUR 1088.4
Proteinase K solution
GZ5904-25ML 25 ml
EUR 418.8
Proteinase K solution
GZ5904-100 100
EUR 870
Proteinase K solution
GZ5904-25 25
EUR 316.4
Proteinase K, 20mg/ml
P2170-002 2X1ml
EUR 181.2
Proteinase K, 20mg/ml
P2170-005 5X1ml
EUR 238.8
Proteinase K (high conc.)
RP02503 100mg Ask for price
PROTEINASE K SOLUTION 10ML
502-PK 10 ml
EUR 286.8
Description: Genomic PCR Grade Proteinase K Solution
PROTEINASE K SOLUTION 25ML
503-PK 25 ml
EUR 602.4
Description: Genomic PCR Grade Proteinase K Solution
Proteinase K, Recombinant, NGS Grade
9247-100 each
EUR 210
Proteinase K, Recombinant, NGS Grade
9247-10G each
EUR 6812.4
Proteinase K, Recombinant, NGS Grade
9247-1G each
EUR 810
Proteinase K, Recombinant, NGS Grade
9247-500 each
EUR 614.4
Proteinase K from Tritirachium album
abx082503-100mg 100 mg
EUR 276
Proteinase K, Recombinant, Standard Grade
9250-100 each
EUR 124.8
Proteinase K, Recombinant, Standard Grade
9250-10G each
EUR 4627.2
Proteinase K, Recombinant, Standard Grade
9250-1G each
EUR 548.4
Proteinase K, Recombinant, Standard Grade
9250-500 each
EUR 314.4
Proteinase K, Recombinant, Standard Grade
9251-100 each
EUR 1266
Proteinase K, Recombinant, Standard Grade
9251-25 each
EUR 352.8
Proteinase K, Recombinant, Standard Grade
9251-5 each
EUR 144
Proteinase K, Recombinant, Molecular Grade
9210-100 each
EUR 144
Proteinase K, Recombinant, Molecular Grade
9210-10G each
EUR 3171.6