Cusao Proteinase K

Proteinase k, recombinant

Proteinase Okay (Lyophilized)

For Basic Digestion of Protein in Pure Samples

  • Lively over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
  • Purified to take away RNase and DNase actions

Secure at Room Temperature and Straightforward to Use

  • Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% prepare at temperatures as rather a lot as 60°C
  • No resuspension or thawing ahead of use
  • Cat.# MC5005, MC5008 provided at a highlight of 20mg/ml

 

Proteinase K, recombinant
Proteinase K, recombinant



Highlights

• Prepared-to-use reply
• Lively in a variety of response circumstances

Capabilities

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

 

Proteinase Okay – one in every of many sometimes used enzymes in molecular biology.

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.



Keep in mind

• The really useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The prepare of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Okay in route of autolysis, will enhance the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Okay
• Secure over an enormous pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum prepare at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.

 

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular prepare.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the best high quality product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.

Decisions

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Excessive prepare and distinctive purity.
  • Lively at excessive temperatures (as rather a lot as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in quite a lot of options.
  • Secure over an enormous pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Capabilities:

  • Extraction of DNA and RNA from fully completely totally different beginning offers.
  • Purification of goal provides from contaminating proteins.
  • Elimination of DNases and RNases all by nucleic acids isolation.

 

Proteinase K
BIO-37039 1000mg Ask for price
Proteinase K
G029 1.0 ml
EUR 142
Proteinase K
GE0990-100MG 100 mg
EUR 94
Proteinase K
GE0990-1G 1 g
EUR 413
Proteinase K
GE0990-250MG 250 mg
EUR 154
Proteinase K
GE0990-25MG 25 mg
EUR 58
Proteinase K
GE0990-500MG 500 mg
EUR 249
Proteinase K
K1037-1 1 ml
EUR 67
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.
Proteinase K
K1037-5 5 ml
EUR 108
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.
Proteinase K
P2180-010 100mg
EUR 182
Proteinase K
P2180-050 500mg
EUR 374
Proteinase K
P2180-100 1g
EUR 523
Proteinase K
Z5050002 100 mg
EUR 171
Description: No genetic downstream application can do without the good old Proteinase K.
Proteinase K protein
30R-2901 500 mg
EUR 425
Description: Purified recombinant Human Proteinase K protein
Proteinase K Antibody
6210-100
EUR 294
Proteinase K Antibody
6210-30T
EUR 146
PROTEINASE K SOLUTION
501-PK 5 ml
EUR 152
Description: Genomic PCR Grade Proteinase K Solution
Proteinase K Solution
BIO-37084 5ml @ 20mg/ml Ask for price
Proteinase K Solution
BIO-37085 5 x 5ml @ 20mg/ml Ask for price
Proteinase K solution
GZ5904-100ML 100 ml
EUR 907
Proteinase K solution
GZ5904-25ML 25 ml
EUR 349
10x Proteinase K
TG4039 0.5ml
EUR 134
Proteinase K - 1g
PB0451 1g
EUR 556.34
  • Product category: Proteins/Enzymes/Proteases
PROTEINASE K SOLUTION 10ML
502-PK 10 ml
EUR 239
Description: Genomic PCR Grade Proteinase K Solution
PROTEINASE K SOLUTION 25ML
503-PK 25 ml
EUR 502
Description: Genomic PCR Grade Proteinase K Solution
Proteinase K, 20mg/ml
P2170-002 2X1ml
EUR 151
Proteinase K, 20mg/ml
P2170-005 5X1ml
EUR 199
Proteinase K, Recombinant, Molecular Grade
9210-100
EUR 120
Proteinase K, Recombinant, Molecular Grade
9210-10G
EUR 2643
Proteinase K, Recombinant, Molecular Grade
9210-1G
EUR 338
Proteinase K, Recombinant, Molecular Grade
9210-500
EUR 262
Proteinase K, Recombinant, Molecular Grade
9210-50G Ask for price
Proteinase K, Recombinant, NGS Grade
9247-100
EUR 175
Proteinase K, Recombinant, NGS Grade
9247-10G
EUR 5677
Proteinase K, Recombinant, NGS Grade
9247-1G
EUR 675
Proteinase K, Recombinant, NGS Grade
9247-500
EUR 512
Proteinase K, Recombinant, Standard Grade
9250-100
EUR 104
Proteinase K, Recombinant, Standard Grade
9250-10G
EUR 3856
Proteinase K, Recombinant, Standard Grade
9250-1G
EUR 457
Proteinase K, Recombinant, Standard Grade
9250-500
EUR 262
Proteinase K, Recombinant, Standard Grade
9251-100
EUR 1055
Proteinase K, Recombinant, Standard Grade
9251-25
EUR 294
Proteinase K, Recombinant, Standard Grade
9251-5
EUR 120
Proteinase K from Tritirachium album
abx082503-100mg 100 mg
EUR 230
  • Shipped within 5-10 working days.
K-Ras Activation Assay
STA-400-K 20 assays
EUR 757
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.
Bacterial DNA Extraction Kit (Proteinase K included)
BA05 5 preps Ask for price
Bacterial DNA Extraction Kit (Proteinase K included)
BA100 100 preps
EUR 138
Bacterial DNA Extraction Kit (Proteinase K included)
BA50 50 preps
EUR 100