Cusao Proteinase K

Proteinase k, recombinant

Proteinase Okay (Lyophilized)

For Basic Digestion of Protein in Pure Samples

  • Lively over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
  • Purified to take away RNase and DNase actions

Secure at Room Temperature and Straightforward to Use

  • Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% prepare at temperatures as rather a lot as 60°C
  • No resuspension or thawing ahead of use
  • Cat.# MC5005, MC5008 provided at a highlight of 20mg/ml

 

Proteinase K, recombinant
Proteinase K, recombinant



Highlights

• Prepared-to-use reply
• Lively in a variety of response circumstances

Capabilities

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

 

Proteinase Okay – one in every of many sometimes used enzymes in molecular biology.

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.



Keep in mind

• The really useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The prepare of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Okay in route of autolysis, will enhance the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Okay
• Secure over an enormous pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum prepare at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.

 

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular prepare.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the best high quality product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.

Decisions

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Excessive prepare and distinctive purity.
  • Lively at excessive temperatures (as rather a lot as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in quite a lot of options.
  • Secure over an enormous pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Capabilities:

  • Extraction of DNA and RNA from fully completely totally different beginning offers.
  • Purification of goal provides from contaminating proteins.
  • Elimination of DNases and RNases all by nucleic acids isolation.

 

Proteinase K
P2180-100 1g
EUR 627.6
Proteinase K
GE0990-100MG 100 mg
EUR 112.8
Proteinase K
GE0990-1G 1 g
EUR 495.6
Proteinase K
GE0990-250MG 250 mg
EUR 184.8
Proteinase K
GE0990-25MG 25 mg
EUR 69.6
Proteinase K
GE0990-500MG 500 mg
EUR 298.8
Proteinase K
G029 1.0 ml
EUR 170.4
Proteinase K
K1037-1 1 ml
EUR 80.4
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.
Proteinase K
K1037-5 5 ml
EUR 129.6
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.
Proteinase K
GE0990-1 1
EUR 379.6
Proteinase K
GE0990-100 100
EUR 63.3
Proteinase K
GE0990-25 25
EUR 27.8
Proteinase K
GE0990-250 250
EUR 123.4
Proteinase K
GE0990-500 500
EUR 217.6
Proteinase K
Z5050002 100 mg
EUR 123
Proteinase K
DE102-01 1 ml
EUR 11.6
Proteinase K
E40KMP1792 20ug
EUR 495
PROTEINASE K
IB05402 200MG
EUR 158.37
Description: (FR)
Proteinase K
EQ023 1mL
EUR 16
Proteinase K
30R-3452 50 mg Ask for price
Description: Proteinase K protein
PROTEINASE K 100MG
IB05400 -
EUR 97.6
Description: (FR)
Proteinase K - 1g
PB0451 1g
EUR 667.61
10x Proteinase K
TG4039 0.5ml
EUR 160.8
Proteinase K protein
30R-2901 500 mg
EUR 510
Description: Purified recombinant Human Proteinase K protein
Proteinase K 100 mg
DD91553 EACH
EUR 201.6
Proteinase K Solution
BIO-37084 5ml @ 20mg/ml Ask for price
Proteinase K Solution
BIO-37085 5 x 5ml @ 20mg/ml Ask for price
Proteinase K Antibody
6210-100 each
EUR 352.8
Proteinase K Antibody
6210-30T each
EUR 175.2
PROTEINASE K SOLUTION
501-PK 5 ml
EUR 182.4
Description: Genomic PCR Grade Proteinase K Solution
Proteinase K solution
GZ5904-100ML 100 ml
EUR 1088.4
Proteinase K solution
GZ5904-25ML 25 ml
EUR 418.8
Proteinase K solution
GZ5904-100 100
EUR 870
Proteinase K solution
GZ5904-25 25
EUR 316.4
Proteinase K, >30U/MG
A3650-100MG 100MG
EUR 88
Description: Biotechnology
Proteinase K, >30U/MG
A3650-1G 1G
EUR 726
Description: Biotechnology
Proteinase K, >30U/MG
A3650-5G 5G
EUR 2332
Description: Biotechnology
Proteinase K, 20mg/ml
P2170-002 2X1ml
EUR 181.2
Proteinase K, 20mg/ml
P2170-005 5X1ml
EUR 238.8
Proteinase K (high conc.)
RP02503 100mg Ask for price
Proteinase K (High Conc.)
E40KMP1793 20ug
EUR 495
Active Proteinase K (PROK)
RPU57762-100ug 100ug
EUR 470.4
Active Proteinase K (PROK)
RPU57762-1mg 1mg
EUR 2184
Active Proteinase K (PROK)
RPU57762-50ug 50ug
EUR 385
PROTEINASE K SOLUTION 5ML
IB05406 -
EUR 144.52
Description: (REF)
PROTEINASE K SOLUTION 10ML
502-PK 10 ml
EUR 286.8
Description: Genomic PCR Grade Proteinase K Solution
PROTEINASE K SOLUTION 25ML
503-PK 25 ml
EUR 602.4
Description: Genomic PCR Grade Proteinase K Solution
Human Proteinase K ELISA Kit
SL2809Hu 96 Tests
EUR 468