Cusao Proteinase K
Proteinase Okay (Lyophilized)
For Basic Digestion of Protein in Pure Samples
- Lively over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
- Purified to take away RNase and DNase actions
Secure at Room Temperature and Straightforward to Use
- Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
- Retains >80% prepare at temperatures as rather a lot as 60°C
- No resuspension or thawing ahead of use
- Cat.# MC5005, MC5008 provided at a highlight of 20mg/ml
Highlights
• Prepared-to-use reply
• Lively in a variety of response circumstances
Capabilities
• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise
Proteinase Okay – one in every of many sometimes used enzymes in molecular biology.
Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.
Keep in mind
• The really useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The prepare of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Okay in route of autolysis, will enhance the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Okay
• Secure over an enormous pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum prepare at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.
Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular prepare.
Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the best high quality product.
Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.
Decisions
- Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
- Excessive prepare and distinctive purity.
- Lively at excessive temperatures (as rather a lot as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in quite a lot of options.
- Secure over an enormous pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
- Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
- Accessible as powder, lyophilized “cake” or liquid.
Capabilities:
- Extraction of DNA and RNA from fully completely totally different beginning offers.
- Purification of goal provides from contaminating proteins.
- Elimination of DNases and RNases all by nucleic acids isolation.
 Proteinase K |
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| P2180-100 | GenDepot | 1g | EUR 627.6 |
Proteinase K |
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| GE0990-100MG | Glentham Life Sciences | 100 mg | EUR 112.8 |
Proteinase K |
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| GE0990-1G | Glentham Life Sciences | 1 g | EUR 495.6 |
Proteinase K |
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| GE0990-250MG | Glentham Life Sciences | 250 mg | EUR 184.8 |
Proteinase K |
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| GE0990-25MG | Glentham Life Sciences | 25 mg | EUR 69.6 |
Proteinase K |
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| GE0990-500MG | Glentham Life Sciences | 500 mg | EUR 298.8 |
Proteinase K |
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| G029 | ABM | 1.0 ml | EUR 170.4 |
Proteinase K |
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| K1037-1 | ApexBio | 1 ml | EUR 80.4 |
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Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease. |
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Proteinase K |
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| K1037-5 | ApexBio | 5 ml | EUR 129.6 |
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Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease. |
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Proteinase K |
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| Z5050002 | Biochain | 100 mg | EUR 123 |
Proteinase K |
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| DE102-01 | Vazyme | 1 ml | EUR 11.6 |
Proteinase K |
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| GE0990-1 | Glentham Life Sciences | 1 | EUR 379.6 |
Proteinase K |
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| GE0990-100 | Glentham Life Sciences | 100 | EUR 63.3 |
Proteinase K |
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| GE0990-25 | Glentham Life Sciences | 25 | EUR 27.8 |
Proteinase K |
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| GE0990-250 | Glentham Life Sciences | 250 | EUR 123.4 |
Proteinase K |
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| GE0990-500 | Glentham Life Sciences | 500 | EUR 217.6 |
 Proteinase K - 1g |
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| PB0451 | Bio Basic | 1g | EUR 667.61 |
 10x Proteinase K |
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| TG4039 | TopoGen | 0.5ml | EUR 160.8 |
 Proteinase K protein |
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| 30R-2901 | Fitzgerald | 500 mg | EUR 510 |
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Description: Purified recombinant Human Proteinase K protein |
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 Proteinase K 100 mg |
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| DD91553 | Scientific Laboratory Supplies | EACH | EUR 201.6 |
 Proteinase K Solution |
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| BIO-37084 | Bioline | 5ml @ 20mg/ml | Ask for price |
Proteinase K Solution |
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| BIO-37085 | Bioline | 5 x 5ml @ 20mg/ml | Ask for price |
 Proteinase K Antibody |
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| 6210-100 | Biovision | each | EUR 352.8 |
Proteinase K Antibody |
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| 6210-30T | Biovision | each | EUR 175.2 |
 PROTEINASE K SOLUTION |
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| 501-PK | Viagen | 5 ml | EUR 182.4 |
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Description: Genomic PCR Grade Proteinase K Solution |
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 Proteinase K solution |
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| GZ5904-100ML | Glentham Life Sciences | 100 ml | EUR 1088.4 |
Proteinase K solution |
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| GZ5904-25ML | Glentham Life Sciences | 25 ml | EUR 418.8 |
Proteinase K solution |
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| GZ5904-100 | Glentham Life Sciences | 100 | EUR 870 |
Proteinase K solution |
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| GZ5904-25 | Glentham Life Sciences | 25 | EUR 316.4 |
 Proteinase K, 20mg/ml |
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| P2170-002 | GenDepot | 2X1ml | EUR 181.2 |
Proteinase K, 20mg/ml |
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| P2170-005 | GenDepot | 5X1ml | EUR 238.8 |
) Proteinase K (high conc.) |
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| RP02503 | Abclonal | 100mg | Ask for price |
 PROTEINASE K SOLUTION 10ML |
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| 502-PK | Viagen | 10 ml | EUR 286.8 |
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Description: Genomic PCR Grade Proteinase K Solution |
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 PROTEINASE K SOLUTION 25ML |
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| 503-PK | Viagen | 25 ml | EUR 602.4 |
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Description: Genomic PCR Grade Proteinase K Solution |
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 Proteinase K, Recombinant, NGS Grade |
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| 9247-100 | Biovision | each | EUR 210 |
Proteinase K, Recombinant, NGS Grade |
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| 9247-10G | Biovision | each | EUR 6812.4 |
Proteinase K, Recombinant, NGS Grade |
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| 9247-1G | Biovision | each | EUR 810 |
Proteinase K, Recombinant, NGS Grade |
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| 9247-500 | Biovision | each | EUR 614.4 |
 Proteinase K from Tritirachium album |
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| abx082503-100mg | Abbexa | 100 mg | EUR 276 |
 Proteinase K, Recombinant, Standard Grade |
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| 9250-100 | Biovision | each | EUR 124.8 |
Proteinase K, Recombinant, Standard Grade |
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| 9250-10G | Biovision | each | EUR 4627.2 |
Proteinase K, Recombinant, Standard Grade |
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| 9250-1G | Biovision | each | EUR 548.4 |
Proteinase K, Recombinant, Standard Grade |
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| 9250-500 | Biovision | each | EUR 314.4 |
Proteinase K, Recombinant, Standard Grade |
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| 9251-100 | Biovision | each | EUR 1266 |
Proteinase K, Recombinant, Standard Grade |
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| 9251-25 | Biovision | each | EUR 352.8 |
Proteinase K, Recombinant, Standard Grade |
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| 9251-5 | Biovision | each | EUR 144 |
 Proteinase K, Recombinant, Molecular Grade |
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| 9210-100 | Biovision | each | EUR 144 |
Proteinase K, Recombinant, Molecular Grade |
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| 9210-10G | Biovision | each | EUR 3171.6 |