pureexo exosome isolation kit
Software program
Isolate pure exosome from cell custom media (supernatant). This bundle yields so much better purity than exosome precipitation methodology. This product is for evaluation use solely.
Cat. No. | BL-P100 PureExo Cell |
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Producer | 101Bio |
Key Choices |
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Particulars | Product Dimension of 10 reactions (2 mL~4 mL medium / response). The yield of each response is 50~200 µL exosome, from which 150~400 µg exosomal protein or 50 ~ 200 ng exosomal RNA will likely be extracted. |
Notes | Storage: maintain all bottles upright , in cool and darkish place. Shelf Life: 12 months. Because of the exosome yield of this bundle is 10 fold better than all completely different kits, you may start with solely 2 mL medium. The utmost medium amount of each response is 4 mL from at most 5 x 105 cells. One PureExo® Column is only for one response. Do not exceed 4 mL medium or 5 x 105 cells to avoid imprecise layer separation and column clogging. |
Storage Temp | Ambient Temperature |
Supply Conditions | Ambient |
FAQ | 1 Could your bundle isolate/purify greater exosome-like vesicles as a lot as 1000 nm in diameter, from custom medium? A: No, our bundle cannot isolate vesicles better than 300nm.2, Does your P101 bundle work for plasma? A: It actually works fully for plasma.Three What’s the minimal amount of serum sample will likely be processed by your PureExo bundle (Cat.#: P101)? A: 50 ul.4 Which bundle must I exploit for my urine and plasma samples? A: Use DiagExo® bundle (Cat.#: P120) to isolate exosome from urine and Use PureExo® bundle for serum (Cat.#: P101) to isolate exosome from serum.5, Can I exploit your bundle to isolate exosome or endothelial microparticles from cultured cells and from plasma? A: Our P100 bundle may be utilized to isolate exosome from each cell custom medium or serum. It is not for cells. 6 Is your exosome prep contaminated with IgG and/or beads? 7, Do your exosome prep incorporates Albumin? 8, Can I exploit your PureExo bundle (Cat. #P100) on our D.discoideum amoeba? 9, What is the composition of the buffer that the last word exosomes prep may be in? Will it have an effect on my downstream assay? 11, Are exosome functionally energetic? 12, Is it very important to utilize glass tubes? 13, Why can’t I see clear fluff middle layer? 14, What biomarker(s) do you utilize to find out / characterize the isolated exosomes? 15, What is the yield of exosome using your kits? 16, Can I exploit heparin or EDTA tube to assemble blood sample if I need to isolate exosome from plasma? |
Description
P100 bundle is for exosome purification / isolation at extreme yield and purity from cell custom media.
- Easy to utilize: No ultra-centrifugation (< 2 hours)
- 10 fold better yield (vs. completely different kits and ultracentrifuge)
- Save worth (vs. antibodies-beads methodology)
- Isolate Pure exosome (>95% purity vs. 25% purity by exosome precipitation methodology)
- Intact exosome (good morphology).
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