Glypican-Mouse Monoclonal Antibody
DESCRIPTION
Common description
Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which can even be present in a secreted type. Just lately, GPC3 was recognized to be a helpful tumor marker for the prognosis of HCC, hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms′ tumor. In sufferers with HCC, GPC3 was over expressed in neoplastic liver tissue and elevated in serum, however was undetectable in regular liver, benign liver, and the serum of wholesome donors.
GPC3 expression was additionally discovered to be greater in HCC liver tissue than in cirrhotic liver or liver with focal lesions akin to dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. Within the context of testicular germ cell tumors, GPC3 expression is up-regulated in sure histologic subtypes, particularly yolk sac tumors and choriocarcinoma. A excessive stage of GPC3 expression has additionally been present in some sorts of embryonal tumors, akin to Wilms′ tumor and hepatoblastoma, with a low or undetectable expression in regular adjoining tissue. Collectively these research point out that GPC3 is a crucial tumor marker.
PROPERTIES
organic supply
mouse
antibody type
tradition supernatant
antibody product kind
major antibodies
clone
1G12, monoclonal
description
For In Vitro Diagnostic Use in Choose Areas (See Chart)
type
buffered aqueous resolution
species reactivity
human
packaging
vial of 0.1 mL focus (261M-94)
vial of 0.5 mL focus (261M-95)
bottle of 1.0 mL predilute (261M-97)
vial of 1.0 mL focus (261M-96)
bottle of seven.0 mL predilute (261M-98)
producer/tradename
Cell Marque™
utility(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
isotype
IgG1
conjugate
unconjugated
management
hepatocellular carcinoma (HCC)
shipped in
moist ice
storage temp.
2-8°C
visualization
cytoplasmic
Glypican Three Monoclonal Antibody (9C2)
Product Particular Info
MA5-17083 targets GPC3 in FACS, ICC, IHC, IF and WB purposes and reveals reactivity with Human and Mouse samples.
The MA5-17083 immunogen is purified recombinant fragment of human GPC3 expressed in E. Coli.
MA5-17083 detects GPC3 which has a predicted molecular weight of roughly 65.5kDa.
Goal Info
GPC3 is a cell floor proteoglycan that bears heparan sulfate. This protein could also be concerned within the suppression/modulation of development within the predominantly mesodermal tissues and organs, and will play a task within the modulation of IGF2 interactions with its receptor and thereby modulate its perform. Members of the glypican-related integral membrane proteoglycan household include a core protein anchored to the cytoplasmic membrane by way of a glycosyl phosphatidylinositol (GPI) linkage.
These proteins might play a task within the management of cell division, development regulation, and tumor predisposition. Deletion mutations in GPC3 are the reason for Simpson-Golabi-Behmel syndrome (SGBS), often known as Simpson dysmorphia syndrome (SDYS). SGBS is a situation characterised by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
Recombinant Anti-Glypican Three antibody
Key options and particulars
- Produced recombinantly (animal-free) for top batch-to-batch consistency and long run safety of provide
- Rabbit monoclonal [SP86] to Glypican 3
- Appropriate for: ICC/IF, Movement Cyt, WB, IHC-P
- Knockout validated
- Reacts with: Human
-
Species reactivity
Reacts with: Human -
Immunogen
Artificial peptide inside Human Glypican Three aa 500 to the C-terminus (C terminal). The precise sequence is proprietary.
Database hyperlink: P51654 -
Optimistic management
- Human liver most cancers tissueThis antibody gave a optimistic end result when used within the following formaldehyde fastened cell traces: HepG2. ICC/IF: HepG2Flow Cyt: HepG2
-
Common notes
This product is a recombinant monoclonal antibody, which presents a number of benefits together with:
- – Excessive batch-to-batch consistency and reproducibility
- – Improved sensitivity and specificity
- – Lengthy-term safety of provide
- – Animal-free manufacturing
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