bioinfor

Galactose Binding-Soluble 3 ELISA Kit

Goose Lectin-Galactose Binding-Soluble three ELISA Equipment from MYBIOSOURCE INC.

Product Identify Goose Lectin-Galactose Binding-Soluble three ELISA Equipment
Description Assay Sort: Sandwich.
Measurement n/a
Focus n/a
Purposes n/a
Different Names [LGALS3; LGALS3 protein; galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35; lectin, galactoside-binding, soluble, 3], [LGALS3; LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2]
Gene, Accession, CAS # [LGALS3], Gene ID: 3958, NCBI: CAG46894.1, UniProt: Q6FGL0
Catalog # MBS018373

Antibodies

We’re dedicated to elevating all of our antibodies in-house. We are able to hint again every lot again to the unique host it was raised from. We even synthesize the antigens used to boost our antibodies. No extra questioning whether or not the antibody you might be utilizing is simply being re-sold below a special label.

 

Rat Lectin-galactose binding-soluble three ELISA Equipment

Producer MyBioSource
Class ELISA
Sort Elisa-Equipment
Particular in opposition to Rat
Quantity 96-Strip-Wells
Merchandise no. MBS722004-96
eClass 6.1 32160605
eClass 9.0 32160605
Sensitivity
1.Zero pg/mL.
Samples
Serum, plasma, Cell Tradition Supernatants, physique fluid and tissue homogenate
Gene Names
Lgals3 ; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Assay Sort
Sandwich
Host/Reactivities
Reactivity: Rat

Affinity® ECL Western Blotting Substrate (fg grade)

bioinfor
bioinfor

Introduction

The Affinity ECL Western Blotting Substrate is a extremely delicate nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. Affinity ECL Western Blotting Substrate permits the detection of femtogram quantities of antigen and permits for straightforward detection of HRP utilizing photographic or different imaging strategies. Blots may be repeatedly uncovered to X-ray movie to acquire optimum outcomes or stripped of the immunodetection reagents and re-probed. The particular formulation of Affinity ECL Substrate makes it the best substitute for different ECL Substrate with out the necessity for extra optimization of assay circumstances.

Essential Product Data

  • AffinityECL Substrate requires extra dilute antibody concentrations than these used with precipitating colorimetric HRP substrates. To optimize antibody concentrations, carry out a scientific dot blot evaluation.
  • Empirical testing is important to find out the suitable blocking reagent for every Western blot system, as crossreactivity of the blocking reagent with antibodies causes nonspecific sign. Blocking buffer additionally impacts system sensitivity.
  • Keep away from utilizing milk as a blocking reagent when utilizing avidin/biotin methods as a result of milk comprises variable quantities of endogenous biotin, which causes excessive background sign.
  • Use adequate volumes of wash buffer, blocking buffer, antibody answer and substrate working answer to cowl blot and be sure that it by no means turns into dry. Utilizing giant blocking and wash buffer volumes minimizes nonspecific sign.
  • For optimum outcomes, use a shaking platform throughout incubation steps.
  • Add Tween™-20 Detergent (closing focus of 0.05-0.1%) to the blocking buffer and all diluted antibody options to attenuate nonspecific sign.
  • Don’t use sodium azide as a preservative for buffers. Sodium azide is an inhibitor of HRP and will intervene with this technique.
  • Don’t deal with membrane with naked fingers. At all times put on gloves or use clear forceps.
  • All tools have to be clear and freed from overseas materials. Metallic gadgets (e.g., scissors) should have no seen indicators of rust. Rust might trigger speckling and excessive background.
  • Publicity to the solar or another intense gentle can hurt the substrate. For greatest outcomes maintain the substrate working answer in an amber bottle and keep away from extended publicity to any intense gentle. Quick-term publicity to typical laboratory lighting won’t hurt the working answer.

Process Abstract

Observe: Antigen and antibody quantities might require optimization.

  1. Dilute the first antibody to5.0-0.5µg/mL.
  2. Dilute the secondary antibody to 1.0-0.067µg/mL.
  3. Combine Detection Reagents 1 and a pair of at a 1:1 ratio and add it to the blot. Incubate blot for 1-60 seconds.
  4. Drain extra reagent. Cowl blot with a transparent plastic sheet protector or clear plastic wrap.
  5. Expose blot to X-ray movie.

Detailed Western Blotting Process

  1. Take away blot from the switch equipment and block nonspecific websites with Blocking Reagent for 60 minutes at room temperature (RT) with shaking. If desired, block in a single day at 2-8ºC with out shaking.
  2. Take away the Blocking Reagent and add the first antibody working dilution. Incubate blot for 1 hour at RT with shaking or in a single day at 2-8°C with out shaking.
  3. Briefly rinse membrane in Wash Buffer two occasions.
  4. Wash membrane by suspending it in Wash Buffer and agitating for ≥ 5 minutes. Substitute Wash Buffer not less than 4-6 occasions. Rising the Wash Buffer quantity, the variety of washes and wash period might assist decrease background sign.
  5. Incubate blot with the HRP-conjugate working dilution for 1 hour at RT with shaking.
  6. Repeat Steps three and Four to take away nonbound HRP-conjugate.

Observe: Membrane MUST be completely washed after incubation with the HRP-conjugate.

  1. Put together the substrate working answer by mixing equal components of Detection Reagents 1 and a pair of. Use 0.125mL Working Answer per cm2 of membrane.

Observe: For greatest outcomes put together working answer instantly earlier than use. The working answer is steady for 1 hour at RT.

  1. Incubate blot with working answer for 1 minute at RT.
  2. Take away blot from working answer and place it in a plastic sheet protector or clear plastic wrap. Use an absorbent tissue to take away extra liquid and to fastidiously press out any bubbles from between the blot and the membrane protector.
  3. Place the protected membrane in a movie cassette with the protein aspect dealing with up. Flip off all lights besides these applicable for X-ray movie publicity (e.g., a crimson safelight).

Observe: Movie should stay dry throughout publicity. For optimum outcomes, carry out the next precautions:

  • Be sure that extra substrate is faraway from the membrane and the membrane protector.
  • Use gloves throughout the complete film-handling course of.
  • By no means place a blot on developed movie, as chemical substances on the movie might cut back sign.
  1. Fastidiously place X-ray movie on prime of the membrane. Carry out a first-time publicity of30 seconds. Range the publicity time to realize optimum outcomes.
  2. Develop movie utilizing applicable growing answer and fixative. If sign is simply too intense, cut back publicity time or strip and re-probe the blot with decreased antibody concentrations.

Storage

Upon receipt retailer at room temperature.

Cat.# Product Identify Measurement
Okay003 Answer Ⅰ 25/50/250 ml
Okay004 Answer Ⅱ 25/50/250 ml

mAb mouse anti-monkey IL-13

CT109 0.5 mg
EUR 260

mAb mouse anti-human IL-13

CT227 0.5 mg
EUR 260

IL-2, Interleukin-2, murine (mouse)

RC232-13 5ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Cytokines

IL-13

E21-C89 10ug
EUR 343

IL-13, Interleukin-13, murine (mouse)

RC232-24 2ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Cytokines

Anti-human IL-13 antibody

STJ15100097 500 µg
EUR 527
Description: This monoclonal antibody is recommended for neutralization of human IL-13 bioactivity.

Anti-human IL-13 antibody

STJ15100099 250 µg
EUR 336
Description: This monoclonal antibody enables sensitive and specific detection of human IL-13 in immunoassays such as ELISpot.

Anti-human IL-13 antibody

STJ15100100 250 µg
EUR 367
Description: This monoclonal antibody enables sensitive and specific detection of human IL-13 in immunoassays such as ELISA.

Anti-human IL-13 antibody

STJ15100101 250 µg
EUR 336
Description: This monoclonal antibody enables sensitive and specific detection of human IL-13 in immunoassays such as ELISA.

Anti-human IL-13 antibody

STJ15100102 250 µg
EUR 367
Description: This monoclonal antibody enables sensitive and specific detection of human IL-13 in immunoassays such as ELISpot.

Anti-IL-13 alpha antibody

STJ93678 200 µl
EUR 197
Description: Rabbit polyclonal to IL-13Ralpha1.

Anti-IL-13 alpha antibody

STJ93679 200 µl
EUR 197
Description: Rabbit polyclonal to IL-13Ralpha2.

Anti-IL-13 alpha antibody

STJ97311 200 µl
EUR 197
Description: Rabbit polyclonal to IL-13Ralpha2.

99999 CAP 13-415

99999-13 1000/pk
EUR 219
Description: Disposable Screw Cap Culture Tubes; DSCCT's, Caps

9998 SCREW CAP 415/13

9998-13 288/pk
EUR 198
Description: General Apparatus; Stoppers

Mouse Interleukin 13, IL-13 ELISA Kit

CSB-E04602m-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse Interleukin 13, IL-13 in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Interleukin 13, IL-13 ELISA Kit

1-CSB-E04602m
  • EUR 603.00
  • EUR 4247.00
  • EUR 2260.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse Interleukin 13, IL-13 in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Interleukin 13 (IL-13) CLIA Kit

abx195852-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Mouse Interleukin 13,IL-13 ELISA Kit

CN-02456M1 96T
EUR 447

Mouse Interleukin 13,IL-13 ELISA Kit

CN-02456M2 48T
EUR 296

Mouse IL-13(Interleukin 13) ELISA Kit

EM0103 96T
EUR 476.25
  • Detection range: 31.25-2000 pg/ml
  • Uniprot ID: P20109
  • Alias: Interleukin-13,T-cell activation protein P600,Il-13
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Mouse Interleukin 13(IL-13)ELISA Kit    

GA-E0041MS-48T 48T
EUR 336

Mouse Interleukin 13(IL-13)ELISA Kit    

GA-E0041MS-96T 96T
EUR 534

IL-13 Interleukin-13 Mouse Recombinant Protein

PROTP20109 Regular: 10ug
EUR 317
Description: Interleukin-13 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 111 amino acids and having a molecular mass of 12.3 kDa. ;The IL-13 is purified by proprietary chromatographic techniques.

Mouse Interleukin 13(IL-13)ELISA Kit

QY-E20834 96T
EUR 361

pAb rabbit anti-human IL-13

CT226 0.5 mg
EUR 184

Anti-human/monkey IL-13 antibody

STJ15100098 250 µg
EUR 367
Description: This monoclonal antibody enables sensitive and specific detection of monkey IL-13 in immunoassays such as ELISA and ELISpot.

IL-13, Interleukin-13, human

RC212-24 2ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Cytokines

Leave a Reply

Your email address will not be published. Required fields are marked *