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Mouse Anti-Nuclear Antigens

Alpha Diagnostic Worldwide Mouse Anti-Nuclear Antigens (ANA/ENA) Ig’s (whole (A+G+M)  ELISA Package, 96 checks, Quantitative

Producer:  Alpha Diagnostic Worldwide

This equipment measure ANA Ig’s (IgG+A+M) in mouse serum. It’s attainable to make use of isotype particular anti-mouse Ig, resembling anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype particular antibodies.

Product Particulars

Properties

Assay kind Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection vary 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Pattern kind &Pattern quantity serum, plasma and different organic fluids; 100μL
Specificity This equipment acknowledges Human IgG in samples. No important cross-reactivity or interference between Human IgG and analogues was noticed.
Reproducibility Each intra-CV and inter-CV are < 10%.
Utility This ELISA equipment applies to the in vitro quantitative willpower of Human IgG concentrations in serum, plasma and different organic fluids.

Check Precept

This ELISA equipment makes use of the Sandwich-ELISA precept. The micro ELISA plate supplied on this equipment has been pre-coated with an antibody particular to Human IgG. Requirements or samples are added to the micro ELISA plate wells and mixed with the particular antibody. Then a biotinylated detection antibody particular for Human IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to every micro plate properly and incubated. Free parts are washed away. The substrate answer is added to every properly. Solely these wells that comprise Human IgG, biotinylated detection antibody and Avidin-HRP conjugate will seem blue in shade. The enzyme-substrate response is terminated by the addition of cease answer and the colour turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD worth is proportional to the focus of Human IgG. You may calculate the focus of Human IgG within the samples by evaluating the OD of the samples to the usual curve.

Package parts & Storage

An unopened equipment could be saved at 2-8℃ for 1 month. If the equipment will not be supposed for use inside 1 month, retailer the parts individually in line with the next circumstances as soon as the equipment is acquired.
Merchandise Specs Storage
Micro ELISA Plate(Dismountable) 96T: Eight wells ×12 strips
48T: Eight wells ×6 strips
-20℃, 6 months
Reference Commonplace 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Shield from mild), 6 months
Reference Commonplace & Pattern Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Shield from mild)
Cease Answer 1 vial, 10 mL 2-8°C
Plate Sealer 5 items
Handbook 1 copy
Certificates of Evaluation 1 copy

Mouse IL-33(Interleukin 33) ELISA Package

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Properties

Assay kind Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection vary 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Pattern kind &Pattern quantity serum, plasma and different organic fluids; 100μL
Specificity This equipment acknowledges Mouse IL-33 in samples. No important cross-reactivity or interference between Mouse IL-33 and analogues was noticed.
Reproducibility Each intra-CV and inter-CV are < 10%.
Utility This ELISA equipment applies to the in vitro quantitative willpower of Mouse IL-33 concentrations in serum, plasma and different organic fluids.

Check Precept

This ELISA equipment makes use of the Sandwich-ELISA precept. The micro ELISA plate supplied on this equipment has been pre-coated with an antibody particular to Mouse IL-33. Requirements or samples are added to the micro ELISA plate wells and mixed with the particular antibody. Then a biotinylated detection antibody particular for Mouse IL-33 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to every micro plate properly and incubated. Free parts are washed away. The substrate answer is added to every properly. Solely these wells that comprise Mouse IL-33, biotinylated detection antibody and Avidin-HRP conjugate will seem blue in shade. The enzyme-substrate response is terminated by the addition of cease answer and the colour turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD worth is proportional to the focus of Mouse IL-33. You may calculate the focus of Mouse IL-33 within the samples by evaluating the OD of the samples to the usual curve.

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