Molecular Biology of Basal and Squamous Cell Carcinomas
The prevalent keratinocyte-derived neoplasms of the pores and skin are basal cell carcinoma and squamous cell carcinoma. Each so-called non-melanoma pores and skin cancers comprise the most typical cancers in people by far. Widespread danger components for each tumor entities embrace solar publicity, DNA restore deficiencies resulting in chromosomal instability, or immunosuppression.
But, basic variations within the growth of the 2 totally different entities have been and are at the moment unveiled. The constitutive activation of the sonic hedgehog signaling pathway by acquired mutations within the PTCH and SMO genes seems to symbolize the early basal cell carcinoma developmental determinant.
Though different signaling pathways are additionally affected, small hedgehog inhibitory molecules evolve as probably the most promising basal cell carcinoma therapy choices systemically in addition to topically in present medical trials. For squamous cell carcinoma growth, mutations within the p53 gene, particularly UV-induced mutations, have been recognized as early occasions.
But, different signaling pathways together with epidermal development issue receptor, RAS, Fyn, or p16INK4a signaling could play important roles in squamous cell carcinoma growth. The improved understanding of the molecular occasions main to totally different tumor entities by de-differentiation of the identical cell sort has begun to pave the way in which for modulating new molecular targets therapeutically with small molecules.
Rising Novel Coronavirus is a International Menace: Perception within the Biology of COVID-19 and its Hijacking Strategy of Hosts’ Cell
The outbreak of novel corona virus (2019-nCoV) has unfold out globally. If we glance again in 1960 when first look of the corona virus (CoV) occurred, it was thought of non-virulent. Forty-two years later, folks grew to become contaminated with an unknown virus in Guangdong province in China, displaying signs of extreme acute respiratory syndrome (SARS), after genomic evaluation, CoV was detected however there was additionally a drastic genomic change in between SARS-CoV and CoV that was present in 1960.
Thereafter, it broke out once more in 2012 because the (MERS-CoV) and 2019 (2019-nCoV). These genomic transformations are related to mutation which favors the CoV for evolution and with higher adaptation using hijacking focused host cells extra appropriately in the direction of quicker transcription and replication, and infect human by transmission by means of direct or oblique contact of the contaminated people by means of inhaling droplets originated by coughing or sneezing in contaminated folks.
CoV begins replicating by a brand new host thus, the potential reason behind the genomic transformation of every new CoV-strain is the higher adaptation and better virulence. On this regards the most recent pressure of extreme acute deficiency syndrome-coronavirus-2 (SARS-CoV-2) might be extra deadly. For correct understanding, on this evaluate, we implicated how CoV binds to host receptors, and we offer temporary introduction of the mutation, replication, transmission and pathogenicity of this virus. All of those phases of coronavirus are very important for his or her distinctive evolution.
Motile cilia genetics and cell biology: massive outcomes from little mice
Our understanding of motile cilia and their position in illness has elevated tremendously over the past twenty years, with important data and perception coming from the evaluation of mouse fashions. Motile cilia kind on particular epithelial cell varieties and usually beat in a coordinated, whip-like method to facilitate the circulation and clearance of fluids alongside the cell floor.
Defects in formation and performance of motile cilia end in major ciliary dyskinesia (PCD), a genetically heterogeneous dysfunction with a well-characterized phenotype however no efficient therapy. Quite a lot of mannequin methods, starting from unicellular eukaryotes to mammals, have offered details about the genetics, biochemistry, and construction of motile cilia.
Nonetheless, with exceptional sources out there for genetic manipulation and developmental, pathological, and physiological evaluation of phenotype, the mouse has risen to the forefront of understanding mammalian motile cilia and modeling PCD. That is evidenced by numerous related mouse strains and an intensive physique of genetic and phenotypic knowledge.
Extra lately, software of revolutionary cell organic methods to those fashions has enabled substantial development in elucidating the molecular and mobile mechanisms underlying the biogenesis and performance of mammalian motile cilia. On this article, we’ll evaluate genetic and cell organic research of motile cilia in mouse fashions and their contributions to our understanding of motile cilia and PCD pathogenesis.
 N-Ras Activation Assay |
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| STA-400-N | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms. |
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 H-Ras Activation Assay Kit, Trial Size |
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| STA-400-H-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms. |
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 K-Ras Activation Assay Kit, Trial Size |
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| STA-400-K-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms. |
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 N-Ras Activation Assay Kit, Trial Size |
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| STA-400-N-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms. |
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 Rac1 Activation Assay |
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| STA-401-1 | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit. |
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 Rac2 Activation Assay |
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| STA-401-2 | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit. |
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 Cdc42 Activation Assay |
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| STA-402 | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit. |
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 RhoA Activation Assay |
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| STA-403-A | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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 RhoB Activation Assay |
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| STA-403-B | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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 RhoC Activation Assay |
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| STA-403-C | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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 Arf1 Activation Assay |
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| STA-407-1 | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit. |
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 Arf6 Activation Assay |
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| STA-407-6 | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit. |
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 Ral Activation Assay |
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| STA-408 | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit. |
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 Ran Activation Assay |
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| STA-409 | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit. |
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 Pan-RAS-IN-1 |
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| HY-101295 | MedChemExpress | 25mg | EUR 1703 |
 Rap1 Activation Assay Kit |
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| STA-406-1 | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit. |
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 Rap2 Activation Assay Kit |
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| STA-406-2 | Cell Biolabs | 20 assays | EUR 757 |
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit. |
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 Rac1 Activation Assay Kit, Trial Size |
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| STA-401-1-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit. |
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 Rac2 Activation Assay Kit, Trial Size |
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| STA-401-2-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit. |
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 Cdc42 Activation Assay Kit, Trial Size |
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| STA-402-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit. |
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 RhoA Activation Assay Kit, Trial Size |
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| STA-403-A-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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 RhoB Activation Assay Kit, Trial Size |
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| STA-403-B-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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 RhoC Activation Assay Kit, Trial Size |
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| STA-403-C-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. |
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 Rac1/Cdc42 Activation Assay Combo Kit |
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| STA-404 | Cell Biolabs | 2 x 20 assays | EUR 1088 |
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Description: Our Rac1/Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of the small GTPase. The precipitated small GTPase is then detected by Western blot using a specific antibody included in the kit. |
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 Rap1 Activation Assay Kit, Trial Size |
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| STA-406-1-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit. |
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 Rap2 Activation Assay Kit, Trial Size |
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| STA-406-2-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit. |
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 Arf1 Activation Assay Kit, Trial Size |
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| STA-407-1-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit. |
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 Arf6 Activation Assay Kit, Trial Size |
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| STA-407-6-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit. |
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 Ral Activation Assay Kit, Trial Size |
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| STA-408-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit. |
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 Ran Activation Assay Kit, Trial Size |
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| STA-409-T | Cell Biolabs | 5 assays | EUR 403 |
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Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit. |
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 RhoA/Rac1/CDC42 Activation Assay Combo Kit |
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| STA-405 | Cell Biolabs | 3 x 10 assays | EUR 1088 |
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Description: Our RhoA/Rac1/Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of the small GTPase. The precipitated small GTPase is then detected by Western blot using a specific antibody included in the kit. |
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) 96-Well Ras Activation ELISA Kit (Colorimetric) |
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| STA-440 | Cell Biolabs | 96 assays | EUR 902 |
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Description: Our 96-Well Ras Activation ELISA Kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody. |
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) 96-Well Ras Activation ELISA Kit (Chemiluminescent) |
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| STA-441 | Cell Biolabs | 96 assays | EUR 902 |
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Description: Our 96-Well Ras Activation ELISA Kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody. |
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 Antibody) Pan-PAX (Pan-PAX) Antibody |
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| abx236136-100ug | Abbexa | 100 ug | EUR 481 |
 RAS antibody |
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| 10R-1779 | Fitzgerald | 100 ul | EUR 349 |
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Description: Mouse monoclonal RAS antibody |
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 RAS Antibody |
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| 31153-100ul | SAB | 100ul | EUR 252 |
RAS Antibody |
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| 31153-50ul | SAB | 50ul | EUR 187 |
 Ras Antibody |
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| 48578-100ul | SAB | 100ul | EUR 333 |
Ras Antibody |
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| 48578-50ul | SAB | 50ul | EUR 239 |
Ras Antibody |
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| 48602-100ul | SAB | 100ul | EUR 333 |
Ras Antibody |
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| 48602-50ul | SAB | 50ul | EUR 239 |
Ras Antibody |
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| 49408-100ul | SAB | 100ul | EUR 333 |
Ras Antibody |
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| 49408-50ul | SAB | 50ul | EUR 239 |
) Rabbit Anti-Human IgG, Antisera (Bulk) |
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| 10119-RAS-BLK | Alpha Diagnostics | 1-10L, bulk | Ask for price |
, Antisera (Bulk)) Rabbit Anti-Llama IgG (H+L chain), Antisera (Bulk) |
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| 30818-RAS-BLK | Alpha Diagnostics | 1-10L, bulk | Ask for price |
) Rabbit Anti-Goat IgG, Antisera (Bulk) |
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| 60319-RAS-BLK | Alpha Diagnostics | 1-10L, bulk | Ask for price |
) Rabbit Anti-Mouse IgG, Antisera (Bulk) |
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| 44319-RAS-BLK | Alpha Diagnostics | 1-10L, bulk | Ask for price |
; Clone PAN-CK (Concentrate)) Cytokeratin, pan (Epithelial Marker); Clone PAN-CK (Concentrate) |
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| RA0430-C.5 | ScyTek Laboratories | 0.5 ml | EUR 300 |
 FOXO1 pan antibody |
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| 70R-37057 | Fitzgerald | 100 ug | EUR 327 |
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Description: Rabbit Polyclonal FOXO1 pan antibody |
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 SSX pan antibody |
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| 70R-50426 | Fitzgerald | 100 ul | EUR 244 |
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Description: Purified Polyclonal SSX pan antibody |
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 NBPF pan antibody |
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| 70R-51031 | Fitzgerald | 100 ul | EUR 244 |
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Description: Purified Polyclonal NBPF pan antibody |
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 PKC pan antibody |
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| 70R-33408 | Fitzgerald | 100 ug | EUR 327 |
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Description: Rabbit polyclonal PKC pan antibody |
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 Actin-pan Antibody |
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| AF0115 | Affbiotech | 200ul | EUR 304 |
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Description: Actin-pan antibody detects endogenous levels of total Actin-pan. |
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 PKC-pan Antibody |
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| AF6197 | Affbiotech | 200ul | EUR 304 |
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Description: PKC-pan Antibody detects endogenous levels of total PKC-pan. |
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 PKC- pan Antibody |
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| ABF6197 | Lifescience Market | 100 ug | EUR 438 |
 pan Cadherin Antibody |
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| ABD2942 | Lifescience Market | 100 ug | EUR 438 |
 pan- AKT Antibody |
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| ABD7208 | Lifescience Market | 100 ug | EUR 438 |
pan Cadherin Antibody |
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| ABD7514 | Lifescience Market | 100 ug | EUR 438 |
 Actin- pan Antibody |
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| ABF0115 | Lifescience Market | 100 ug | EUR 438 |
Actin- pan Antibody |
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| ABF5288 | Lifescience Market | 100 ug | EUR 438 |
 Keratin pan antibody |
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| 20R-2530 | Fitzgerald | 250 uL | EUR 510 |
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Description: Rabbit polyclonal Keratin pan antibody |
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 Synuclein pan antibody |
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| 70R-30766 | Fitzgerald | 100 ug | EUR 327 |
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Description: Rabbit polyclonal Synuclein pan antibody |
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 Actin pan antibody |
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| 70R-31095 | Fitzgerald | 100 ug | EUR 327 |
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Description: Rabbit polyclonal Actin pan antibody |
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 H3 Pan Antibody |
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| 6806-50 | Biovision | EUR 316 | |
 pan Cytokeratin antibody |
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| 10R-2060 | Fitzgerald | 100 ul | EUR 403 |
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Description: Mouse monoclonal pan Cytokeratin antibody |
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 Cytokeratin Pan antibody |
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| 10R-2096 | Fitzgerald | 100 ul | EUR 403 |
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Description: Mouse monoclonal Cytokeratin Pan antibody |
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 pan Keratin antibody |
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| 10R-2442 | Fitzgerald | 50 ug | EUR 405 |
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Description: Mouse monoclonal pan Keratin antibody |
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 LDH Pan antibody |
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| 10-1335 | Fitzgerald | 1 mg | EUR 259 |
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Description: Mouse monoclonal LDH Pan antibody |
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LDH Pan antibody |
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| 10-1336 | Fitzgerald | 1 mg | EUR 259 |
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Description: Mouse monoclonal LDH Pan antibody |
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 pan Endothelium antibody |
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| 10R-6386 | Fitzgerald | 100 ug | EUR 284 |
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Description: Mouse monoclonal pan Endothelium antibody |
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 pan Cadherin antibody |
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| 10R-7890 | Fitzgerald | 100 ug | EUR 322 |
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Description: Mouse monoclonal pan Cadherin antibody |
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 Actin-pan antibody |
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| 10R-A106a | Fitzgerald | 100 ug | EUR 553 |
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Description: Mouse monoclonal Actin-pan antibody |
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pan Cadherin Antibody |
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| DF2942 | Affbiotech | 200ul | EUR 304 |
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Description: pan Cadherin Antibody detects endogenous levels of total pan Cadherin. |
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 pan-AKT Antibody |
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| DF7208 | Affbiotech | 200ul | EUR 304 |
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Description: pan-AKT Antibody detects endogenous levels of total pan-AKT. |
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pan Cadherin Antibody |
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| DF7514 | Affbiotech | 200ul | EUR 304 |
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Description: pan Cadherin Antibody detects endogenous levels of total pan Cadherin. |
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 Pan Methylated Lysine |
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| DF7728 | Affbiotech | 200ul | EUR 304 |
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Description: Pan Methylated Lysine Rabbit pAbdetects endogenous levels of total Pan Methylated Lysine Rabbit pAb. |
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 Pan myristoylation Antibody |
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| 1-CSB-PA885152 | Cusabio |
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Description: A polyclonal antibody against Pan myristoylation. Recognizes Pan myristoylation from ALL. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-10000, ELISA:1:10000 |
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 anti-pan-Synuclein |
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| LF-PA0194 | Abfrontier | 100 ul | EUR 334 |
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Description: Rabbit polyclonal to pan-Synuclein |
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 Pan Keratin antibody |
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| PAab09935 | Lifescience Market | 100 ug | EUR 386 |
Pan myristoylation Antibody |
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| 1-CSB-PA465729 | Cusabio |
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Description: A polyclonal antibody against Pan myristoylation. Recognizes Pan myristoylation from ALL. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-10000, ELISA:1:10000 |
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 Pan-AKT Antibody |
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| 20-abx125375 | Abbexa |
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Pan myristoylation Antibody |
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| 20-abx322788 | Abbexa |
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Pan myristoylation Antibody |
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| 20-abx322789 | Abbexa |
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 Pan Ubiquitin Antibody |
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| abx415347-50ug | Abbexa | 50 ug | EUR 565 |
 pan ADH Antibody |
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| abx433090-200ul | Abbexa | 200 ul | EUR 384 |
 Antibody) SHANK (pan) Antibody |
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| abx440129-100ug | Abbexa | 100 ug | EUR 578 |
 Antibody) SAPAP (pan) Antibody |
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| abx440140-100ug | Abbexa | 100 ug | EUR 578 |
 Antibody) Ankyrin (pan) Antibody |
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| abx440246-100ug | Abbexa | 100 ug | EUR 578 |
SHANK (pan) Antibody |
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| abx440410-100ug | Abbexa | 100 ug | EUR 578 |
SAPAP (pan) Antibody |
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| abx440421-100ug | Abbexa | 100 ug | EUR 578 |
Ankyrin (pan) Antibody |
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| abx440527-100ug | Abbexa | 100 ug | EUR 578 |
SHANK (pan) Antibody |
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| abx440691-100ug | Abbexa | 100 ug | EUR 578 |
SAPAP (pan) Antibody |
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| abx440702-100ug | Abbexa | 100 ug | EUR 578 |
