MOLECULAR INVESTIGATION AND PHYLOGENETIC ANALYSIS OF ANAPLASMOSIS IN DOGS
Anaplasmosis is a widespread vector-borne illness affecting canines, and Anaplasma platys is the key etiological agent of the illness. The research examines anaplasmosis molecular prevalence, associated threat elements, and alteration of hematological variables in Anaplasma-affected canines. A complete of 150 blood samples have been collected from canines within the district of Lahore, Pakistan. The samples have been screened with PCR focusing on the 16S rRNA gene of Anaplasma. Sequencing of samples that have been discovered constructive after performing PCR was performed. A questionnaire was developed to gather epidemiological knowledge on topic canines, and the data was analyzed with a logistic regression mannequin utilizing SPSS.
The present research revealed an 11.34% (17/150) prevalence of anaplasmosis in canines primarily based on PCR detection. Tick infestation, earlier tick historical past, home hygiene, and tick management standing have been main threat elements linked with illness incidence. Crimson blood cell depend, packed cell quantity, hemoglobin, and platelet depend have been decreased considerably (P < 0.05) in Anaplasma-infected canines. Phylogenetically, the two isolates of the present research clustered collectively, and that cluster was similar to A. platys isolates from India, Malaysia, and Thailand.
New association of three genera of fish tapeworms (Cestoda: Proteocephalidae) in catfishes (Siluriformes) from the Neotropical Area: taxonomic implications of molecular phylogenetic analyses
Tapeworms (Cestoda: Proteocephalidae) are the dominant element of communities of intestinal parasites in pimelodid and different catfishes (Siluriformes) from South America. Regardless that these parasites have been studied intensively over a couple of century, molecular taxonomy and phylogenetics have questioned their morphology-based classification, thus elevating doubts in regards to the systematic worth of traits generally used to circumscribe particular person taxa. Within the current research, members of three morphologically well-characterized genera of proteocephalids from pimelodid (Hemisorubim platyrhynchos and Sorubim lima) and auchenipterid (Ageneiosus inermis) catfishes from the Paraná or Amazon River basins have been subjected to DNA sequencing of the massive subunit nuclear ribosomal RNA (lsrDNA) and full mitochondrial cytochrome c oxidase subunit I (COI).
Phylogenetic analyses revealed the sister relationship between Manaosia bracodemoca and Mariauxiella piscatorum, and amongst Mariauxiella pimelodi and Ageneiella brevifilis. Consequently, Mar. piscatorum and A. brevifilis are transferred to Manaosia and Mariauxiella, respectively, as Manaosia piscatorum n. comb. and Mariauxiella brevifilis n. comb., and the genus Ageneiella is suppressed. Diagnoses of Manaosia and Mariauxiella are amended. In addition, the current research revealed misidentification of tapeworms whose sequences are deposited within the GenBank database.
Molecular characterization and phylogenetic analyses of Tripartiella macrosoma Basson and Van As, 1987 and Tripartiella obtusa Ergens and Lom, 1970 primarily based on 18S rRNA gene sequence knowledge
Within the current research, we supplied the primary 18S rRNA gene sequence knowledge of two Tripartiella species, Tripartiella macrosoma Basson and Van As, 1987 and Tripartiella obtusa Ergens and Lom, 1970, which have been remoted from Tachysurus fulvidraco (Richardson, 1846) and Hemibarbus maculatus Bleeker, 1871 in Chongqing, China, respectively. Morphologically, each species fall throughout the morphometry vary of the unique descriptions and are similar to the unique populations within the general look of the adhesive disc. Tripartiella macrosoma might be simply distinguished from the opposite Tripartiella species by possessing the denticle with an extended strip and conspicuously inclined backward blade and a sturdy and quick ray.
Tripartiella obtusa is principally characterised by a broad blade and a comparatively lengthy ray. Phylogenetically, T. macrosoma clustered with Trichodinella myakkae (Mueller, 1937) Raabe, 1950 and additional with Trichodinella sp., which was sister to a bunch that features 4 populations of Trichodinella epizootica (Raabe, 1950) Šrámek-Hušek, 1953; lastly, they fashioned a small clade with T. obtusa. This consequence advised that T. macrosoma had a better relationship with Trichodinella spp. than with T. obtusa and T. obtusa diverged sooner than T. macrosoma and Trichodinella spp. By combining morphological and molecular knowledge, the polyphyletic traits of Tripartiella and Trichodinella have been additional analyzed, and the outcomes revealed that the validity of the genus Tripartiella is uncertain.
Canine Visceral Leishmaniasis in São Paulo, Brazil, the Most Populous Metropolis of South America: Isolation, Molecular Analysis, and Phylogenetic Inferences
Background and Goals: Canine visceral leishmaniasis impacts canines, the primary city reservoirs, which favor the transmission and enlargement of this zoonotic illness in areas with excessive anthropization course of and human density. We investigated the occurence of Leishmania infatum primarily based in molecular analysis, and phylogenetic evaluation of isolates obtained from canines in metropolitan area of São Paulo. Strategies: A complete of 201 canines have been examined by parasitological and molecular analysis. Phylogenetic evaluation primarily based sequences from SSUrDNA and gGAPDH genes have been carried out. Outcomes: The parasitological analysis revealed 5% (10/201) of positivity, and the sequences obtained from seven isolates have been clustered with L. infantum in phylogentic evaluation primarily based on SSUrDNA and gGAPDH genes. A complete of 24.9% (50/201) of canines have been constructive in molecular analysis primarily based on cathepsin L-like marker. Interpretation and Conclusion: In accordance with this research, it’s essential to implement a surveillance coverage of visceral leishmaniasis, intensifying the actions of analysis, prevention, and management of this zoonosis.
Morphological Description, Phylogenetic and Molecular Evaluation of Dirofilaria immitis Remoted from Canine within the Northwest of Iran
Background: Dirofilariasis is a globally distributed arthropod-borne parasitic illness of primarily canids and felids. We evaluated to increase the data of morpho-molecular traits and outer ultrastructure of Dirofilaria immitis remoted from Northwest of Iran.
Strategies: General, 67 filarial worms together with 41 females and 26 males parasites have been collected from the cardiovascular system of the 43 stray canines in Meshkinshar, Ardebil Province, Northwest of Iran in 2017, and subjected to gentle and scanning electron microscopy (SEM) in addition to carmine alum staining for morpho-molecular and identification. Molecular strategies have been used for affirmation of morphological findings by sequencing of Cyto-chrome c oxidase subunit I (cox1) gene.
Outcomes: The partial DNA sequencing of cox1 gene of grownup parasites confirmed appreciable homology and shut proximity to the beforehand remoted from Kerman and Meshkinshahr, Iran. The bottom genetic variation and the very best intra-species variability was present in D. immitis and Dirofilaria repens, respectively. No similarity was recognized between D. immitis nucleotide sequence and Wolbachia species as its endosymbiont micro organism.
Conclusion: The SEM method is a superb device for differential recognition of the parasite floor morphology and molecular strategies might differentiate and establish Dirofilaria spp.