Hepatitis B virus (HBV) is a non-cytopathic, hepatotropic virus with the potential to trigger a persistent an infection, in the end resulting in cirrhosis and hepatocellular carcinoma.

Over the previous 4 many years, the fundamental ideas of HBV gene expression and replication in addition to the viral and host determinants governing an infection end result have been largely uncovered. Whereas HBV seems to induce little or no innate immune activation, the adaptive immune response mediates each viral clearance in addition to liver illness.

Right here, we assessment our present data on the immunobiology and pathogenesis of HBV an infection, focusing specifically on the position of CD8⁺ T cells and on a number of current breakthroughs that problem present dogmas.

For instance, we now belief that HBV integration into the host genome usually serves as a related supply of hepatitis B floor antigen (HBsAg) expression throughout continual an infection, presumably triggering dysfunctional T cell responses and favouring detrimental immunopathology.

Additional, the distinctive haemodynamics and anatomy of the liver – and the modifications they regularly endure throughout illness development to liver fibrosis and cirrhosis – profoundly affect T cell priming, differentiation and performance.

We additionally focus on why therapeutic approaches that restrict the intrahepatic inflammatory processes triggered by HBV-specific T cells is likely to be surprisingly useful for sufferers with continual an infection.

Schistosomes within the Lung: Immunobiology and Alternative

Schistosome an infection is a significant trigger of world morbidity, significantly in sub-Saharan Africa. Nonetheless, there is no such thing as a efficient vaccine for this main uncared for tropical illness, and re-infection routinely happens after chemotherapeutic therapy. Following invasion via the pores and skin, larval schistosomula enter the circulatory system and migrate via the lung earlier than maturing to maturity within the mesenteric or urogenital vasculature.

 

Eggs launched from grownup worms can turn into trapped in numerous tissues, with resultant inflammatory responses resulting in hepato-splenic, intestinal, or urogenital illness – processes which have been extensively studied lately.

 

In distinction, though lung pathology can happen in each the acute and continual phases of schistosomiasis, the mechanisms underlying pulmonary illness are significantly poorly understood. In continual an infection, egg-mediated fibrosis and vascular destruction can result in the formation of portosystemic shunts via which eggs can embolise to the lungs, the place they will set off granulomatous illness.

 

Acute schistosomiasis, or Katayama syndrome, which is primarily evident in non-endemic people, happens throughout pulmonary larval migration, maturation, and preliminary egg-production, usually involving fever and a cough with an accompanying immune cell infiltrate into the lung. Importantly, lung migrating larvae will not be only a reason behind irritation and pathology however are a key goal for future vaccine design.

 

Nonetheless, vaccine efforts are hindered by a restricted understanding of what constitutes a protecting immune response to larvae. On this assessment, we discover the present understanding of pulmonary immune responses and inflammatory pathology in schistosomiasis, highlighting necessary unanswered questions and areas for future analysis.

 

Immunobiology and nanotherapeutics of extreme acute respiratory syndrome 2 (SARS-CoV-2): a present replace

The emergence of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) constitutes essentially the most important international public well being problem in a century. It has reignited analysis curiosity in coronavirus.

 

• Whereas little data is out there, analysis is at the moment in progress to comprehensively perceive the final biology and immune response mechanism in opposition to SARS-CoV-2. The spike proteins (S protein) of SARS-CoV-2 carry out a vital operate in viral an infection institution. ACE2 and TMPRSS2 play a pivotal position in viral entry. “
• Upon viral entry, the launched pro-inflammatory proteins (cytokines and chemokines) trigger the migration of the T cells, monocytes, and macrophages to the an infection website.
• IFNϒ launched by T cells initiates a loop of pro-inflammatory suggestions. The inflammatory state might additional improve with a rise in immune dysfunction liable for the an infection’s development.
• A therapy strategy that stops ACE2-mediated viral entry and reduces inflammatory response is a vital therapeutic intervention technique, and nanomaterials and their conjugates are promising candidates. Nanoparticles can inhibit viral entry and replication.
• Nanomaterials have additionally discovered utility in focused drug supply and likewise in growing a vaccine in opposition to SARS-CoV-2. Right here, we briefly summarize the origin, transmission, and medical options of SARS-CoV-2. We then mentioned the immune response mechanisms of SARS-CoV-2.
• Lastly, we additional mentioned nanotechnology’s potentials as an intervention technique in opposition to SARS-CoV-2 an infection. All these understandings will likely be essential in growing therapeutic methods in opposition to SARS-CoV-2.

PD-1 immunobiology in glomerulonephritis and renal cell carcinoma

Background: Programmed cell demise protein (PD)-1 receptors and ligands on immune cells and kidney parenchymal cells assist preserve immunological homeostasis within the kidney. Dysregulated PD-1:PD-L1 binding interactions happen throughout the pathogenesis of glomerulopathies and renal cell carcinoma (RCC). The regulation of those molecules within the kidney is necessary to PD-1/PD-L1 immunotherapies that deal with RCC and should induce glomerulopathies as an opposed occasion.

Strategies: The expression and performance of PD-1 molecules on immune and kidney parenchymal cells had been reviewed within the wholesome kidney, PD-1 immunotherapy-induced nephrotoxicity, glomerulopathies and RCC.

Outcomes: PD-1 and/or its ligands are expressed on kidney macrophages, dendritic cells, lymphocytes, and renal proximal tubule epithelial cells. Vitamin D3, glutathione and AMP-activated protein kinase (AMPK) regulate hypoxic cell indicators concerned within the expression and performance of PD-1 molecules.

These pathways are altered in kidney illness and are linked to the manufacturing of vascular endothelial development issue, erythropoietin, adiponectin, interleukin (IL)-18, IL-23, and chemokines that bind CXCR3, CXCR4, and/or CXCR7.

These components are differentially produced in glomerulonephritis and RCC and could also be necessary biomarkers in sufferers that obtain PD-1 therapies and/or develop glomerulonephritis as an opposed occasion CONCLUSION: By evaluating the capabilities of the PD-1 axis in glomerulopathies and RCC, we recognized related chemokines concerned within the recruitment of immune cells and distinct mediators in T cell differentiation.

 

The expression and performance of PD-1 and PD-1 ligands in diseased tissue and significantly on double-negative T cells and parenchymal kidney cells wants continued exploration. The doable regulation of the PD-1 axis by vitamin D3, glutathione and/or AMPK cell indicators could also be necessary to kidney illness and the PD-1 immunotherapeutic response.

293AAV Cell Line
AAV-100	Cell Biolabs	1 vial	EUR 340
Description: N/A

293LTV Cell Line
LTV-100	Cell Biolabs	1 vial	EUR 340
Description: N/A

293RTV Cell Line
RV-100	Cell Biolabs	1 vial	EUR 340

MCF7 [MCF-7] Cell Line
CL-0149	Elabscience Biotech	1×10⁶ cells/vial	EUR 420
Description: Homo sapiens, Human

293/GFP Cell Line
AKR-200	Cell Biolabs	1 vial	EUR 388
Description: Fluorescence

T47D/GFP Cell Line
AKR-208	Cell Biolabs	1 vial	EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line
AKR-209	Cell Biolabs	1 vial	EUR 388
Description: Fluorescence

HeLa/GFP Cell Line
AKR-213	Cell Biolabs	1 vial	EUR 388
Description: Fluorescence

293/Cas9 Cell Line
AKR-5110	Cell Biolabs	1 vial	EUR 460

HeLa/Cas9 Cell Line
AKR-5111	Cell Biolabs	1 vial	EUR 460

NIH3T3/GFP Cell Line
AKR-214	Cell Biolabs	1 vial	EUR 388
Description: Fluorescence

NIH3T3/Cas9 Cell Line
AKR-5104	Cell Biolabs	1 vial	EUR 460

MCF-7-LUC cells
S0006002	Addexbio	One Frozen vial	EUR 420

OVCAR-5/RFP Cell Line
AKR-254	Cell Biolabs	1 vial	EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

PKCa Stable Expressing MCF-7 (MCF-7/PKCa20) Cell Line
T6115	ABM	1x10^6 cells / 1.0 ml	EUR 3950

Platinum-E Retroviral Packaging Cell Line, Ecotropic
RV-101	Cell Biolabs	1 vial	EUR 648

Platinum-GP Retroviral Packaging Cell Line, Pantropic
RV-103	Cell Biolabs	1 vial	EUR 648

Platinum-A Retroviral Packaging Cell Line, Amphotropic
RV-102	Cell Biolabs	1 vial	EUR 648

Total Protein - Murine Embryonic Stem Cell Line D3
CBA-305	Cell Biolabs	500 ?g	EUR 414
Description: Isolated from mouse ES-D3 cell line Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

MCF 10A Cell Line
CL-0525	Elabscience Biotech	1×10⁶ cells/vial	EUR 500
Description: Homo sapiens, Human

cDNA - Human Tumor Cell Line: MCF 7
C1255830	Biochain	40 reactions	EUR 311.5

Flavopiridol-Resistant MCF-7 Cell Line (FLV100)
T8033	ABM	1x10^6 cells / 1.0 ml	EUR 2950

Flavopiridol-Resistant MCF-7 Cell Line (FLV1000)
T8035	ABM	1x10^6 cells / 1.0 ml	Ask for price

Luc-Akt-PH Stable MCF7 Cell Line
T3160	ABM	1x10^6 cells / 1.0 ml	EUR 3950

Genomic DNA - Human Tumor Cell Line: MCF 7
D1255830	Biochain	100 ug	EUR 207.2

Exosome derived from human breast cancer, noninvasive cell line (MCF-7 cell line)
Exo-CH06	Creative BioMart	Each	EUR 1249.5
Description: Exosome

AAV2-Luc Control Virus
AAV-320	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 2.

AAV1-Luc Control Virus
AAV-321	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 1.

AAV3-Luc Control Virus
AAV-323	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 3.

AAV4-Luc Control Virus
AAV-324	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 4.

AAV5-Luc Control Virus
AAV-325	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 5.

AAV6-Luc Control Virus
AAV-326	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 6.

Tissue, Section, Human Disease, Human Tumor Cell Line, MCF-7 (Paraffin)
MBS640859-5Slides	MyBiosource	5Slides	EUR 510

Tissue, Section, Human Disease, Human Tumor Cell Line, MCF-7 (Paraffin)
MBS640859-5x5Slides	MyBiosource	5x5Slides	EUR 2145

GAS(Luc) Report Cell Line-HeLa
ABC-RC099D	AcceGen	1 vial	Ask for price
Description: The GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of IFN gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the IFN gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNγ first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind IFN gamma-activated sites (GAS) in the promoter of IFNγ inducible genes, including luciferase reporter gene.

GAL4 Report Cell Line(Luc)-HEK293
ABC-RC113D	AcceGen	1 vial	Ask for price
Description: The GAL4 Reporter (Luc) andndash; HEK293 Cell Line contains a firefly luciferase gene under the control of a multimerized GAL4 upstream activation sequence (UAS) stably integrated into HEK293 cells. The cell line does not contain any exogenous activators of the GAL4 reporter.

NFAT Reporter (Luc) - Jurkat Cell Line
60621	BPS Bioscience	2 vials	EUR 2295
Description: The NFAT Reporter - Jurkat Cell Line contains a firefly luciferase gene under the control of the_x000D_NFAT response element stably integrated into Jurkat cells. This cell line has been validated for_x000D_response to thapsigargin, ionomycin, and phorbol 12-myristate 13-acetate (PMA). It is useful as_x000D_a control cell line for other NFAT reporter cell lines expressing various immune checkpoint_x000D_receptors.

GAL4 Reporter (Luc)-HEK293 Cell Line
60656	BPS Bioscience	2 vials	EUR 1095
Description: The GAL4 Reporter (Luc) - HEK293 Cell Line contains a firefly luciferase gene under the control of a multimerized GAL4 upstream activation sequence (UAS) stably integrated into HEK293 cells. The cell line does not contain any exogenous activators of the GAL4 reporter and can be used alongside BPS Cat. #60655 as a control.

Foxp3(Luc) Report Cell Line-Jurkat
ABC-RC106D	AcceGen	1 vial	Ask for price
Description: Human Foxp3 luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter.

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes
EXOP-100A-1	SBI	50 ug	EUR 409

STAT5 Reporter (Luc) - Ba/F3 Cell line
79772	BPS Bioscience	2 vials	EUR 2275
Description: The STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)
CBA-325	Cell Biolabs	96 assays	EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)
CBA-325-5	Cell Biolabs	5 x 96 assays	EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

IRF Reporter (Luc) - THP-1 Cell line
79858	BPS Bioscience	2 vials	EUR 1810
Description: The Interferon Regulatory Factor (IRF) reporter (Luc)-THP-1 cell line is designed to study the activation and signaling of Cytosolic DNA Sensors (CDS) in human monocytic cell line THP-1. It contains a firefly luciferase gene driven by multimerized ISRE (Interferon Stimulated Response Element) located upstream of the minimal TATA promoter. _x000D_The cGAS-STING pathway acts to detect cytosolic DNA and induce an immune response. Briefly, upon binding DNA, the protein cGAS (cyclic GMP-AMP Synthase) triggers reaction of GTP and ATP to form cGAMP. cGAMP binds to STING (Stimulator of Interferon Genes) which triggers phosphorylation of IRF3 via TBK1. IRF3 can then bind to interferon-stimulated responsive elements (ISRE) in the nucleus and leads to IFN-α/β production. The IRF reporter (Luc)-THP-1 cell line is highly responsive to STING and CDS ligands.

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric
CBA-135	Cell Biolabs	96 assays	EUR 596

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric
CBA-135-5	Cell Biolabs	5 x 96 assays	EUR 2592

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric
CBA-140	Cell Biolabs	96 assays	EUR 624

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric
CBA-140-5	Cell Biolabs	5 x 96 assays	EUR 2700

NF-κB Reporter (Luc) - HCT116 Cell Line
60623	BPS Bioscience	2 vials	EUR 1095
Description: NF- B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κ B transcription factors bind to the DNA response elements to induce transcription of the luciferase gene._x000D_The NF- κB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-κB signaling in response to stimulants such as the cytokines TNF and IL-1β, pathogen-associated molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern (DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for establishing cell-based screens for inhibitors that target specific NF-κB stimulating molecules. This cell line can be further modified to allow investigation of downstream NF-κB activities as a result of targeted genetic mutation(s).

NF-κB reporter (Luc) - HEK293 Cell line
60650	BPS Bioscience	2 vials	EUR 1365
Description: The NF-κB reporter (Luc) HEK293 cell line is designed to monitor nuclear factor Kappa B (NF-κB) activity. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. The cell line has been functionally validated in response to human TNF-α, IL-1β, and IL-17.

GR-GAL4 Report Cell Line(Luc)-HEK293
ABC-RC114D	AcceGen	1 vial	Ask for price
Description: The Glucocorticoid Receptor Pathway GAL4 Reporter (Luc) – HEK293 Cell Line contains a firefly luciferase gene under the control of glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR) stably integrated into HEK293 cells. This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of DXMS and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.

NF-kB reporter (Luc) - HEK293 Cell line
GWB-PS76F8	GenWay Biotech	2X10(6)cells	Ask for price

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size
CBA-135-T	Cell Biolabs	24 assays	EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Tissue, Total Protein, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinima)
MBS657022-1mg	MyBiosource	1mg	EUR 565

Tissue, Total Protein, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinima)
MBS657022-5x1mg	MyBiosource	5x1mg	EUR 2315

Tissue, Membrane Protein, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinima)
MBS639777-01mg	MyBiosource	0.1mg	EUR 570

Tissue, Membrane Protein, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinima)
MBS639777-5x01mg	MyBiosource	5x0.1mg	EUR 2345

STAT3 Reporter (Luc) - HEK293 Cell line (Puromycin)
79800-P	BPS Bioscience	2 vials	EUR 3730
Description: The STAT3 Reporter (Luc)-HEK293 cell line is designed for monitoring STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines and growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

Luciferase Stable 17L3C (17L3C-luc) Cell Line
T6240	ABM	1x10^6 cells / 1.0 ml	EUR 3950

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size
CBA-140-T	Cell Biolabs	24 assays	EUR 547.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line
60628	BPS Bioscience	2 vials	EUR 7645
Description: Human Foxp3 luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter.

NF- κB Reporter (Luc) - Raw 264.7 Cell line
79978	BPS Bioscience	2 vials	EUR 2045
Description: The NF-κB reporter (Luc)-Raw 264.7 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

PAI-1 Report Cell Line (Luc)-Mv1 Lu
ABC-RC199D	AcceGen	1 vial	Ask for price
Description: PAI-1 Reporter (Luc)-Mv1 Lu cell line is designed for monitoring transforming growth factor β (TGF-β)-induced plasminogen activator inhibitor-1 (PAI-1) expression. Transforming growth factor-β (TGF-β) is a potent regulator of cellular differentiation, proliferation, migration, and protein expression.PAI-1 Reporter (Luc) –Mv1 Lu cell line contains a firefly luciferase gene under the control of PAI-1 responsive elements stably integrated into Mv1 Lu (NBL-7) cells, showing TGF-β pathway response. This cell line is validated for the TGF-β response to the induction of PAI-1 gene expression through luciferase activity.

NF- κB Reporter (Luc) - THP-1 Cell Line
79645	BPS Bioscience	2 vials	EUR 1900
Description: The NF-κB reporter (Luc)-THP-1 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

Collagen-based Cell Contraction Assay
CBA-201	Cell Biolabs	24 assays	EUR 348

PAI-1 Reporter (Luc) - Mv1 Lu Cell Line
60544	BPS Bioscience	2 vials	EUR 3595
Description: PAI-1 Reporter (Luc)-Mv1 Lu cell line is designed for monitoring transforming growth factor β (TGF-β)-induced plasminogen activator inhibitor-1 (PAI-1) expression. Transforming growth factor-β (TGF-β) is a potent regulator of cellular differentiation, proliferation, migration, and protein expression._x000D__x000D_PAI-1 Reporter (Luc) -Mv1 Lu cell line contains a firefly luciferase gene under the control of PAI-1 responsive elements stably integrated into Mv1 Lu (NBL-7) cells, showing TGF-β pathway response. This cell line is validated for the TGF-β response to the induction of PAI-1 gene expression through luciferase activity. _x000D_

NF-κB reporter (Luc) - NIH/3T3 Cell line
79469	BPS Bioscience	2 vials	EUR 1900
Description: The NF-κB reporter (Luc)-NIH/3T3 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

NF-κB Reporter (Luc) - CHO-K1 Cell Line
60622	BPS Bioscience	2 vials	EUR 1095
Description: An NF-κB luciferase reporter construct is stably integrated into the genome of CHO-K1 cells. The firefly luciferase gene is controlled by the NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene._x000D_The NF-κB-luciferase / CHO-K1 cell line is suitable for monitoring the activity of NF-κB transcription factor through luminescence readout.). This cell line responds to human cytokine IL-1β, responds moderately to human TNF, and does not respond to human IFN-λ (2 µg/ml). Reducing the amount of serum during incubation period may increase the sensitivity to cytokines. Since CHO-K1 cells do not express endogenous human proteins, this cell line provides an excellent platform to enable exogenous expression of a protein of interest to study its downstream effect on NF-κB signaling.

Radius 384-Well Cell Migration Assay
CBA-127	Cell Biolabs	384 assays	EUR 721.2
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay
CBA-127-5	Cell Biolabs	5 x 384 wells	EUR 2802
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CD40/NF-κB Report Cell Line(Luc) - HEK293
ABC-RC111D	AcceGen	1 vial	Ask for price
Description: Recombinant HEK293 cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5). Expression is confirmed by real-time qPCR and Western Blot. This NF-ĸB luciferase reporter construct is stably integrated into the genome. The firefly luciferase gene is controlled by 4 copies of NF-andkappa;B response element located upstream of the TATA promoter. Following activation by human CD40 ligand, NF-ĸB transcription factor binds to the DNA response elements to induce transcription of the luciferase gene.

HIF-1 Alpha Cell Based ELISA Kit
CBA-281	Cell Biolabs	96 assays	EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

CytoSelect™ MTT Cell Proliferation Assay
CBA-252	Cell Biolabs	960 assays	EUR 268

STAT5 Reporter (Luc)- U937 Cell Line (GM-CSF)
79941	BPS Bioscience	2 vials	EUR 1980
Description: The STAT5 Reporter (Luc)-U937 cell line is designed for monitoring STAT5 signal transduction pathway in the U937 cell line. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter. After activation by GM-CSF, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

NF-κB Reporter (Luc) - A549 Stable Cell Line
60625	BPS Bioscience	2 vials	EUR 1915
Description: NF-κB luciferase reporter construct is stably integrated into the genome of A549 cells. The firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.

Radius™ 24-Well Cell Migration Assay
CBA-125	Cell Biolabs	24 assays	EUR 356
Description: Excepted Quantities

Radius™ 24-Well Cell Migration Assay
CBA-125-5	Cell Biolabs	5 x 24 assays	EUR 1516
Description: Excepted Quantities

Radius™ 96-Well Cell Migration Assay
CBA-126	Cell Biolabs	96 assays	EUR 416
Description: Excepted Quantities

Radius™ 96-Well Cell Migration Assay
CBA-126-5	Cell Biolabs	5 x 96 assays	EUR 1788
Description: Excepted Quantities

Radius™ 48-Well Cell Migration Assay
CBA-5037	Cell Biolabs	48 assays	EUR 372
Description: Excepted Quantities

Radius™ 48-Well Cell Migration Assay
CBA-5037-5	Cell Biolabs	5 x 48 assays	EUR 1592
Description: Excepted Quantities

Human Mcf-7 Whole Cell Lysate
LYSATE0024	BosterBio	200ug	EUR 180
Description: This cell lysate is prepared from human mcf-7 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Tissue, Total RNA, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinoma), BioGenomics
MBS638573-005mg	MyBiosource	0.05mg	EUR 485

Tissue, Total RNA, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinoma), BioGenomics
MBS638573-5x005mg	MyBiosource	5x0.05mg	EUR 1960

Tamoxifen-resistant MCF-7 (MCF-7/TAM1) Cells
T8216	ABM	1x10^6 cells / 1.0 ml	EUR 1350

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)
CBA-250	Cell Biolabs	10 mL	EUR 490.8
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect™ BrdU Cell Proliferation ELISA Kit
CBA-251	Cell Biolabs	96 assays	EUR 380
Description: Excepted Quantities

MCF-7 cells
C0006008	Addexbio	One Frozen vial	EUR 420

MCF-7 Cells
T8978	ABM	1x10^6 cells / 1.0 ml	EUR 650

CytoSelect™ 96-well Cell Transformation Assay
CBA-130	Cell Biolabs	96 assays	EUR 532

CytoSelect™ 96-well Cell Transformation Assay
CBA-130-5	Cell Biolabs	5 x 96 assays	EUR 2280

Tissue, Genomic DNA, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinima), BioGenomics
MBS654437-01mg	MyBiosource	0.1mg	EUR 505

Tissue, Genomic DNA, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinima), BioGenomics
MBS654437-5x01mg	MyBiosource	5x0.1mg	EUR 2125

CytoSelect™ Cell Viability and Cytotoxicity Assay
CBA-240	Cell Biolabs	96 assays	EUR 256

CytoSelect 24-well Laminin Cell Invasion, Colorimetric
CBA-110-LN	Cell Biolabs	12 assays	EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect™ 24-Well Cell Co-Culture System
CBA-160	Cell Biolabs	24 assays	EUR 308

CytoSelect™ 24-Well Cell Co-Culture System
CBA-160-5	Cell Biolabs	5 x 24 assays	EUR 1344

CytoSelect™ 48-Well Cell Contraction Assay Kit
CBA-5021	Cell Biolabs	48 assays	EUR 484

CytoSelect 384-well Cell Transformation Assay, Fluorometric
CBA-145	Cell Biolabs	384 assays	EUR 1208.4
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.