Hepatitis B virus (HBV) is a non-cytopathic, hepatotropic virus with the potential to trigger a persistent an infection, in the end resulting in cirrhosis and hepatocellular carcinoma.

Over the previous 4 many years, the fundamental ideas of HBV gene expression and replication in addition to the viral and host determinants governing an infection end result have been largely uncovered. Whereas HBV seems to induce little or no innate immune activation, the adaptive immune response mediates each viral clearance in addition to liver illness.

Right here, we assessment our present data on the immunobiology and pathogenesis of HBV an infection, focusing specifically on the position of CD8⁺ T cells and on a number of current breakthroughs that problem present dogmas.

For instance, we now belief that HBV integration into the host genome usually serves as a related supply of hepatitis B floor antigen (HBsAg) expression throughout continual an infection, presumably triggering dysfunctional T cell responses and favouring detrimental immunopathology.

Additional, the distinctive haemodynamics and anatomy of the liver – and the modifications they regularly endure throughout illness development to liver fibrosis and cirrhosis – profoundly affect T cell priming, differentiation and performance.

We additionally focus on why therapeutic approaches that restrict the intrahepatic inflammatory processes triggered by HBV-specific T cells is likely to be surprisingly useful for sufferers with continual an infection.

Schistosomes within the Lung: Immunobiology and Alternative

Schistosome an infection is a significant trigger of world morbidity, significantly in sub-Saharan Africa. Nonetheless, there is no such thing as a efficient vaccine for this main uncared for tropical illness, and re-infection routinely happens after chemotherapeutic therapy. Following invasion via the pores and skin, larval schistosomula enter the circulatory system and migrate via the lung earlier than maturing to maturity within the mesenteric or urogenital vasculature.

 

Eggs launched from grownup worms can turn into trapped in numerous tissues, with resultant inflammatory responses resulting in hepato-splenic, intestinal, or urogenital illness – processes which have been extensively studied lately.

 

In distinction, though lung pathology can happen in each the acute and continual phases of schistosomiasis, the mechanisms underlying pulmonary illness are significantly poorly understood. In continual an infection, egg-mediated fibrosis and vascular destruction can result in the formation of portosystemic shunts via which eggs can embolise to the lungs, the place they will set off granulomatous illness.

 

Acute schistosomiasis, or Katayama syndrome, which is primarily evident in non-endemic people, happens throughout pulmonary larval migration, maturation, and preliminary egg-production, usually involving fever and a cough with an accompanying immune cell infiltrate into the lung. Importantly, lung migrating larvae will not be only a reason behind irritation and pathology however are a key goal for future vaccine design.

 

Nonetheless, vaccine efforts are hindered by a restricted understanding of what constitutes a protecting immune response to larvae. On this assessment, we discover the present understanding of pulmonary immune responses and inflammatory pathology in schistosomiasis, highlighting necessary unanswered questions and areas for future analysis.

 

Immunobiology and nanotherapeutics of extreme acute respiratory syndrome 2 (SARS-CoV-2): a present replace

The emergence of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) constitutes essentially the most important international public well being problem in a century. It has reignited analysis curiosity in coronavirus.

 

• Whereas little data is out there, analysis is at the moment in progress to comprehensively perceive the final biology and immune response mechanism in opposition to SARS-CoV-2. The spike proteins (S protein) of SARS-CoV-2 carry out a vital operate in viral an infection institution. ACE2 and TMPRSS2 play a pivotal position in viral entry. “
• Upon viral entry, the launched pro-inflammatory proteins (cytokines and chemokines) trigger the migration of the T cells, monocytes, and macrophages to the an infection website.
• IFNϒ launched by T cells initiates a loop of pro-inflammatory suggestions. The inflammatory state might additional improve with a rise in immune dysfunction liable for the an infection’s development.
• A therapy strategy that stops ACE2-mediated viral entry and reduces inflammatory response is a vital therapeutic intervention technique, and nanomaterials and their conjugates are promising candidates. Nanoparticles can inhibit viral entry and replication.
• Nanomaterials have additionally discovered utility in focused drug supply and likewise in growing a vaccine in opposition to SARS-CoV-2. Right here, we briefly summarize the origin, transmission, and medical options of SARS-CoV-2. We then mentioned the immune response mechanisms of SARS-CoV-2.
• Lastly, we additional mentioned nanotechnology’s potentials as an intervention technique in opposition to SARS-CoV-2 an infection. All these understandings will likely be essential in growing therapeutic methods in opposition to SARS-CoV-2.

PD-1 immunobiology in glomerulonephritis and renal cell carcinoma

Background: Programmed cell demise protein (PD)-1 receptors and ligands on immune cells and kidney parenchymal cells assist preserve immunological homeostasis within the kidney. Dysregulated PD-1:PD-L1 binding interactions happen throughout the pathogenesis of glomerulopathies and renal cell carcinoma (RCC). The regulation of those molecules within the kidney is necessary to PD-1/PD-L1 immunotherapies that deal with RCC and should induce glomerulopathies as an opposed occasion.

Strategies: The expression and performance of PD-1 molecules on immune and kidney parenchymal cells had been reviewed within the wholesome kidney, PD-1 immunotherapy-induced nephrotoxicity, glomerulopathies and RCC.

Outcomes: PD-1 and/or its ligands are expressed on kidney macrophages, dendritic cells, lymphocytes, and renal proximal tubule epithelial cells. Vitamin D3, glutathione and AMP-activated protein kinase (AMPK) regulate hypoxic cell indicators concerned within the expression and performance of PD-1 molecules.

These pathways are altered in kidney illness and are linked to the manufacturing of vascular endothelial development issue, erythropoietin, adiponectin, interleukin (IL)-18, IL-23, and chemokines that bind CXCR3, CXCR4, and/or CXCR7.

These components are differentially produced in glomerulonephritis and RCC and could also be necessary biomarkers in sufferers that obtain PD-1 therapies and/or develop glomerulonephritis as an opposed occasion CONCLUSION: By evaluating the capabilities of the PD-1 axis in glomerulopathies and RCC, we recognized related chemokines concerned within the recruitment of immune cells and distinct mediators in T cell differentiation.

 

The expression and performance of PD-1 and PD-1 ligands in diseased tissue and significantly on double-negative T cells and parenchymal kidney cells wants continued exploration. The doable regulation of the PD-1 axis by vitamin D3, glutathione and/or AMPK cell indicators could also be necessary to kidney illness and the PD-1 immunotherapeutic response.

cDNA from Human Tumor Cell Line: MCF 7
C1255830	Biochain	40 reactions	EUR 451.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Mcf-7 Whole Cell Lysate
LYSATE0024	BosterBio	200ug	EUR 180
Description: This cell lysate is prepared from human mcf-7 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

MCF 7 Membrane Lysate
XBL-10442	ProSci	0.1 mg	EUR 619.8
Description: MCF 7 (Human breast Adenocarcinima) cell membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The MCF 7 (Human breast Adenocarcinima) cell was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

MCF-7 Nuclear Extract
X12002	EpiGentek	1000 µg	Ask for price

Genomic DNA from Human Tumor Cell Line: MCF 7
D1255830	Biochain	100 ug	EUR 291.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein from Human Tumor Cell Line: MCF 7
P1255830	Biochain	1 mg	EUR 256.8
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Tumor Cell Line: MCF 7
P3255830	Biochain	0.1 mg	EUR 342
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7
T2255830	Biochain	5 slides	EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total RNA from Human Tumor Cell Line: MCF 7
R1255830-50	Biochain	50 ug	EUR 232.8
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

MCF-7 Nuclear Extract (H2O2)
1642-100	Biovision		EUR 248.4

Human MCF-7 (breast cancer) Cell Nuclear Extract
HCL-2016	Alpha Diagnostics	100ug	EUR 255.6

AAV2-Luc Control Virus
AAV-320	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 2.

AAV1-Luc Control Virus
AAV-321	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 1.

AAV3-Luc Control Virus
AAV-323	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 3.

AAV4-Luc Control Virus
AAV-324	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 4.

AAV5-Luc Control Virus
AAV-325	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 5.

AAV6-Luc Control Virus
AAV-326	Cell Biolabs	50 ?L	EUR 1221.6
Description: Luciferase control virus of AAV serotype 6.

Human AsPC1 / Luciferase Cell Line
SC062-Luc	GenTarget	2 x 106 cell/ml x 1ml	EUR 1800
Description: Firefly luciferase expression stable cell line in Human AsPC1 cells with Puromycin resistance

mouse MOPC315 / Luciferase Cell Line
SC063-Luc	GenTarget	2 x 106 cell/ml x 1ml	EUR 2670
Description: Firefly luciferase expression stable cell line in mouse MOPC315 cells with Puromycin resistance

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)
MCF7-100	Alpha Diagnostics	100 ug	EUR 196.8

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)
MCF7-50	Alpha Diagnostics	50 ug	EUR 153.6

A549 / Luciferase (Puromycin) stable cell line
SC043-Luc	GenTarget	2 x 106 cell/ml x 1ml	EUR 1104
Description: Luciferase (firefry) expression stable cell line in A549 human cancer cell line with Puromycin marker.

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes
EXOP-100A-1	SBI	50 ug	EUR 560.4

Human PANC-1 / Luciferase (Puro) Cell Line
SC068-Luc	GenTarget	2 x 106 cell/ml x 1ml	EUR 2670
Description: Firefly luciferase expression stable cell line in Human PANC-1 cells with Puromycin resistance

MCF-10A
C0006015	Addexbio	One Frozen vial	EUR 560.4

Human MCF-7 (breast cancer) Whole Cell Lysate, Hydrogen Peroxide Stimulated
HCL-2014	Alpha Diagnostics	100ug	EUR 255.6

293AAV Cell Line
AAV-100	Cell Biolabs	1 vial	EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293AD Cell Line
AD-100	Cell Biolabs	1 vial	EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293LTV Cell Line
LTV-100	Cell Biolabs	1 vial	EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line
RV-100	Cell Biolabs	1 vial	EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

293/GFP Cell Line
AKR-200	Cell Biolabs	1 vial	EUR 686.4
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line
AKR-208	Cell Biolabs	1 vial	EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line
AKR-209	Cell Biolabs	1 vial	EUR 686.4
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line
AKR-213	Cell Biolabs	1 vial	EUR 686.4
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line
AKR-214	Cell Biolabs	1 vial	EUR 686.4
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line
AKR-5104	Cell Biolabs	1 vial	EUR 686.4

293/Cas9 Cell Line
AKR-5110	Cell Biolabs	1 vial	EUR 686.4

HeLa/Cas9 Cell Line
AKR-5111	Cell Biolabs	1 vial	EUR 686.4

Anti-Cytokeratin 7
DB051RTU-7	DB Biotech	7 ml	EUR 244.8
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

pT7- Luc
PVT10670	Lifescience Market	2 ug	EUR 361.2

pSBE- Luc
PVT10816	Lifescience Market	2 ug	EUR 361.2

pTRE3G- LUC
PVT10818	Lifescience Market	2 ug	EUR 361.2

pAP1- Luc
PVT10819	Lifescience Market	2 ug	EUR 361.2

pHSE- Luc
PVT10820	Lifescience Market	2 ug	EUR 319.2

pGRE- luc
PVT10821	Lifescience Market	2 ug	EUR 319.2

pCRE- Luc
PVT10825	Lifescience Market	2 ug	EUR 361.2

pViperin- Luc
PVT10826	Lifescience Market	2 ug	EUR 361.2

pTA- Luc
PVT10827	Lifescience Market	2 ug	EUR 361.2

pTAL- Luc
PVT10829	Lifescience Market	2 ug	EUR 361.2

pIRF3- Luc
PVT10830	Lifescience Market	2 ug	EUR 361.2

p53- Luc
PVT10836	Lifescience Market	2 ug	EUR 319.2

pBV-Luc
PVT12245	Lifescience Market	2 ug	EUR 843.6

pMR-Luc
PVT14074	Lifescience Market	2 ug	EUR 843.6

OVCAR-5/RFP Cell Line
AKR-254	Cell Biolabs	1 vial	EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

NF-kB/293/GFP-Luc Transcriptional Reporter Cell Line
TR860A-1	SBI	>2 x 10^6 cells	EUR 3915.6

Recombinant Human Galectin-7
7-00442	CHI Scientific	2µg	Ask for price

Recombinant Human Galectin-7
7-00443	CHI Scientific	10µg	Ask for price

Recombinant Human Galectin-7
7-00444	CHI Scientific	1mg	Ask for price

Recombinant Human Interleukin-7
7-00895	CHI Scientific	2µg	Ask for price

Recombinant Human Interleukin-7
7-00896	CHI Scientific	10µg	Ask for price

Recombinant Human Interleukin-7
7-00897	CHI Scientific	1mg	Ask for price

Recombinant Mouse Interleukin-7
7-00904	CHI Scientific	2µg	Ask for price

Recombinant Mouse Interleukin-7
7-00905	CHI Scientific	10µg	Ask for price

Recombinant Mouse Interleukin-7
7-00906	CHI Scientific	1mg	Ask for price

Recombinant Human Kallikrein-7
7-03034	CHI Scientific	2µg	Ask for price

Recombinant Human Kallikrein-7
7-03035	CHI Scientific	10µg	Ask for price

Recombinant Human Kallikrein-7
7-03036	CHI Scientific	100µg	Ask for price

Recombinant ProMatrix Metalloproteinase-7
7-03478	CHI Scientific	5µg	Ask for price

Recombinant ProMatrix Metalloproteinase-7
7-03479	CHI Scientific	20µg	Ask for price

Recombinant ProMatrix Metalloproteinase-7
7-03480	CHI Scientific	1mg	Ask for price

Individual Reaction Mix 7
G065-7	ABM	200 reactions	EUR 200.4

Recombinant Human Interleukin-7, Saccharomyces
7-00898	CHI Scientific	2µg	Ask for price

Recombinant Human Interleukin-7, Saccharomyces
7-00899	CHI Scientific	10µg	Ask for price

Recombinant Human Interleukin-7, Saccharomyces
7-00900	CHI Scientific	1mg	Ask for price

Recombinant Human Matrix Metalloproteinase-7
7-03154	CHI Scientific	5µg	Ask for price

Recombinant Human Matrix Metalloproteinase-7
7-03155	CHI Scientific	20µg	Ask for price

Recombinant Human Matrix Metalloproteinase-7
7-03156	CHI Scientific	1mg	Ask for price

Lung Lysate (7 Days Old)
1402-7	ProSci	0.1 mg	EUR 229.2
Description: Lung tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (7 Days Old)
1403-7	ProSci	0.1 mg	EUR 229.2
Description: Brain tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (7 Days Old)
1404-7	ProSci	0.1 mg	EUR 229.2
Description: Liver tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (7 Days Old)
1405-7	ProSci	0.1 mg	EUR 229.2
Description: Kidney tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (7 Days Old)
1406-7	ProSci	0.1 mg	EUR 229.2
Description: Spleen tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (7 Days Old)
1409-7	ProSci	0.1 mg	EUR 229.2
Description: Thymus tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate (7 Day Old)
1415-7	ProSci	0.1 mg	EUR 229.2
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (7 Days Old)
1419-7	ProSci	0.1 mg	EUR 229.2
Description: Skin tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Eye Lysate (7 Days Old)
1420-7	ProSci	0.1 mg	EUR 229.2
Description: Eye tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

ICP Quality Control Standard 7
QC-7	Scientific Laboratory Supplies	125ML	EUR 176.7

Platinum-E Retroviral Packaging Cell Line, Ecotropic
RV-101	Cell Biolabs	1 vial	EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic
RV-102	Cell Biolabs	1 vial	EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic
RV-103	Cell Biolabs	1 vial	EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

pUC57-Tac-Luc
PVT18215	Lifescience Market	2 ug	EUR 309.6

p21-Luc Plasmid
PVTB00035-2c	Lifescience Market	2 ug	EUR 427.2

IgK- IFN- luc
PVT10425	Lifescience Market	2 ug	EUR 319.2

pNFAT- TA- Luc
PVT10808	Lifescience Market	2 ug	EUR 319.2

pE2F- TA- Luc
PVT10809	Lifescience Market	2 ug	EUR 319.2

pISRE- TA- Luc
PVT10810	Lifescience Market	2 ug	EUR 319.2

pGAS- TA- Luc
PVT10811	Lifescience Market	2 ug	EUR 319.2

pP53- TA- luc
PVT10814	Lifescience Market	2 ug	EUR 361.2

pStat3- TA- luc
PVT10815	Lifescience Market	2 ug	EUR 361.2