
Immunobiology and immunotherapy of HCC: spotlight on innate and innate-like immune cells
Immune-based therapies similar to immune checkpoint inhibitors have revolutionized the systemic therapy of varied most cancers sorts. The therapeutic software of monoclonal antibodies focusing on inhibitory pathways similar to programmed cell death-1(PD-1)/programmed cell loss of life ligand 1 (PD-L1) and CTLA-Four to cells of the adaptive immune system has just lately been proven to generate significant enchancment within the medical final result of hepatocellular carcinoma (HCC).
However, present immunotherapeutic approaches induce sturdy responses in solely a subset of HCC sufferers. Since immunologic mechanisms similar to continual irritation on account of continual viral hepatitis or alcoholic and nonalcoholic fatty liver illness play an important position within the initiation, growth, and development of HCC, you will need to perceive the underlying mechanisms shaping the distinctive tumor microenvironment of liver most cancers.
The liver is an immunologic organ with massive populations of innate and innate-like immune cells and is uncovered to bacterial, viral, and fungal antigens by way of the gut-liver axis. Right here, we summarize and spotlight the position of those cells in liver most cancers and suggest methods to therapeutically goal them.
We additionally focus on present immunotherapeutic methods in HCC and description latest advances in our understanding of how the therapeutic potential of those brokers could be enhanced.
Peripheral blood T lymphocytosis in thymoma: an perception into immunobiology
Objective: Peripheral blood T lymphocytosis (PBTL) is a uncommon, but poorly understood manifestations of thymoma, which is postulated to be linked with autoimmune/paraneoplastic manifestations similar to myasthenia gravis (MG), pure crimson cell aplasia (PRCA), and so on.; extra generally encountered on this neoplasm.
Technique: We intention to explain the flowcytometric immunophenotypic information of PBTL in a 43-year-old male; 6 months after profitable completion of chemoradiotherapy (CT/RT) for a big, invasive, and metastatic kind B1 thymoma; and current a complete assessment of all such circumstances reported over final 42 years (N = 21) (1977-2019).
Consequence: A bigger dimension of the tumors (≥ 10 cm), presence of native invasion and/or distant metastasis, and sort B (cortical or lymphocyte wealthy) histology have been extra prone to be related to PBTL.
Tumors related to MG/PRCA (N = 9/21) are likely to have decrease PBTL in comparison with these with out such manifestations; and PBTL subsided following thymectomy with or with out CT/RT. Immunophenotypic evaluation of PB revealed a CD8 + > CD4 + mature (naïve) polyclonal T cells resembling late cortical thymocytes.
Conclusion: Thymic intratumoral microenvironment may affect incidence PBTL which will have a pathophysiologic hyperlink to the growth of autoimmune manifestations. Immunophenotypic traits of peripheral blood lymphoid cells ought to be the clue for correct characterization and to keep away from a misdiagnosis of a lymphoproliferative neoplasm.
Immunobiology of Acquired Resistance to Ticks
Ticks are blood-sucking arthropods of nice significance within the medical and veterinary fields worldwide. They’re thought of second solely to mosquitos as vectors of pathogenic microorganisms that may trigger severe infectious issues, such as Lyme borreliosis and tick-borne encephalitis.
Exhausting (Ixodid) ticks feed on host animals for a number of days and inject saliva along with pathogens to hosts throughout blood feeding. Some animal species can purchase resistance to blood-feeding by ticks after a single or repeated tick infestation, leading to decreased weights and numbers of engorged ticks or the loss of life of ticks in subsequent infestations. Importantly, this acquired tick resistance (ATR) can cut back the danger of pathogen transmission from pathogen-infected ticks to hosts.
That is the premise for the event of tick antigen-targeted vaccines to forestall tick infestation and tick-borne ailments. Accumulation of basophils is detected within the tick re-infested pores and skin lesion of animals exhibiting ATR, and the ablation of basophils abolishes ATR in mice and guinea pigs, illustrating the crucial position for basophils within the expression of ATR.
On this assessment article, we offer a complete overview of latest advances in our understanding of the mobile and molecular mechanisms accountable for the event and manifestation of ATR, with a selected deal with the position of basophils.

T-cell immunobiology and cytokine storm of COVID-19
2019 coronavirus illness (COVID-19) presents as a newly acknowledged pneumonia and will quickly progress into acute respiratory misery syndrome which has caused a world pandemic. Till now, no healing remedy has been strongly beneficial for COVID-19 aside from customized supportive care.
T cells and virus-specific T cells are important to guard in opposition to virus an infection, together with COVID-19. Delayed immune reconstitution (IR) and cytokine storm (CS) stay severe obstacles for the remedy of COVID-19.
Most COVID-19 sufferers, particularly amongst aged sufferers, had marked lymphopenia and elevated neutrophils, however T cell counts in extreme COVID-19 sufferers surviving the illness steadily restored later.
Elevated pro-inflammatory cytokines, significantly IL-6, IL-10, IL-2, and IL-17, and exhausted T cells are present in peripheral blood and the lungs. It means that Thymosin α1 and adoptive COVID-19-specific T cells might enhance IR whereas convalescent plasma, IL-6 blockade, mesenchymal stem cells, and corticosteroids might suppress CS.
Extra medical research on this discipline worldwide are urgently warranted to pave the best way for remedy of COVID-19 sooner or later.
In response to a necessity for a normal catalog of genome variation to deal with the large-scale sampling designs required by affiliation research, gene mapping and evolutionary biology, the Nationwide Middle for Biotechnology Data (NCBI) has established the dbSNP database [S.T.Sherry, M.Ward and K. Sirotkin (1999) Genome Res., 9, 677-679]. Submissions to dbSNP will probably be built-in with different sources of knowledge at NCBI similar to GenBank, PubMed, LocusLink and the Human Genome Venture information. The entire contents of dbSNP can be found to the general public at
Fluorescent proteins are genetically encoded, simply imaged reporters essential in biology and biotechnology. When a protein is tagged by fusion to a fluorescent protein, interactions between fluorescent proteins can undesirably disturb focusing on or operate. Sadly, all wild-type yellow-to-red fluorescent proteins reported to this point are obligately tetrameric and sometimes poisonous or disruptive.
The primary true monomer was mRFP1, derived from the Discosoma sp. fluorescent protein “DsRed” by directed evolution first to extend the pace of maturation, then to interrupt every subunit interface whereas restoring fluorescence, which cumulatively required 33 substitutions.
Though mRFP1 has already confirmed extensively helpful, a number of properties might bear enchancment and extra colours can be welcome. We report the following technology of monomers. The most recent crimson model matures extra utterly, is extra tolerant of N-terminal fusions and is over tenfold extra photostable than mRFP1. Three monomers with distinguishable hues from yellow-orange to red-orange have greater quantum efficiencies.
![]() Fluorguard Antifade Mounting Medium |
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F001 | Insitus Biotechnologies | 1 ml | EUR 103.2 |
![]() Fluorescent Mounting Medium with PI, 18 ml |
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E20-18 | IHC World | - | EUR 165 |
![]() Fluazifop solution 10 µg/ml in ethyl acetate |
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GW2901-2 | Glentham Life Sciences | 2 | EUR 126.2 |
![]() 1000 ug/mL Fluorenone in Methanol |
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S-4332 | Scientific Laboratory Supplies | 1ML | EUR 114 |
![]() DiagPoly Plain Fluorescent Polystyrene Particles, Pink, 0.1-0.3 µm |
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DNM-L012 | Creative Diagnostics | 2 mL | EUR 788.4 |
![]() DiagPoly Plain Fluorescent Polystyrene Particles, Yellow, 0.1-0.3 µm |
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DNM-L002 | Creative Diagnostics | 2 mL | EUR 788.4 |
![]() DiagPoly Plain Fluorescent Polystyrene Particles, Purple, 0.1-0.3 µm |
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DNM-L015 | Creative Diagnostics | 2 mL | EUR 788.4 |
![]() DiagPoly Amine Fluorescent Polystyrene Particles, Yellow, 0.09-0.3 µm |
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DNM-L056 | Creative Diagnostics | 2 mL | EUR 832.8 |
![]() DiagPoly Carboxyl Fluorescent Polystyrene Particles, Yellow, 0.09-0.3 µm |
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DNM-L063 | Creative Diagnostics | 2 mL | EUR 789.6 |
![]() DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 0.1-0.3 µm |
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DNM-L009 | Creative Diagnostics | 2 mL | EUR 788.4 |
![]() DiagPoly Plain Fluorescent Polystyrene Particles, Sky Blue, 0.1-0.3 µm |
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DNM-L019 | Creative Diagnostics | 2 mL | EUR 788.4 |
![]() Annexin V-Alexa Fluor488/PI Apoptosis Detection Kit |
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E28LS4001-100 | EnoGene | 100T | EUR 793.65 |
![]() Annexin V-Alexa Fluor488/PI Apoptosis Detection Kit |
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E28LS4001-20 | EnoGene | 20T | EUR 279.37 |
![]() Annexin V-Alexa Fluor488/PI Apoptosis Detection Kit |
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E28LS4001-50 | EnoGene | 50T | EUR 507.94 |
![]() Annexin V-Alexa Fluor647/PI Apoptosis Detection Kit |
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E28LS4002-100 | EnoGene | 100T | EUR 793.65 |
![]() Annexin V-Alexa Fluor647/PI Apoptosis Detection Kit |
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E28LS4002-20 | EnoGene | 20T | EUR 279.37 |
![]() Annexin V-Alexa Fluor647/PI Apoptosis Detection Kit |
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E28LS4002-50 | EnoGene | 50T | EUR 507.94 |
![]() PAH Std Fluorene 2000ug/mL in Toluene |
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REPAH106 | Scientific Laboratory Supplies | 1ML | EUR 58.36 |
![]() PAH Std Fluoranthene 1000ug/mL in DCM |
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REPAH158 | Scientific Laboratory Supplies | 1ML | EUR 59.67 |
![]() PAH Std Fluoranthene 2000ug/mL in DCM |
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REPAH159 | Scientific Laboratory Supplies | 1ML | EUR 73.11 |
![]() DiagPoly Plain Fluorescent Polystyrene Particles, Light Yellow, 0.1-0.3 µm |
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DNM-L005 | Creative Diagnostics | 2 mL | EUR 788.4 |
![]() DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.09-0.3 µm |
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DNM-L071 | Creative Diagnostics | 2 mL | EUR 788.4 |
![]() PAH Std Fluoroanthene 2000ug/mL in Toluene |
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REPAH105 | Scientific Laboratory Supplies | 1ML | EUR 58.36 |
![]() Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit |
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DLSM0244 | DL Develop | 100 Assays | Ask for price |
Description: Detection phosphatidylserine on the outer leaflet of the cell membrane using flow cytometry. |
![]() Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit |
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DLSM0245 | DL Develop | 100 Assays | Ask for price |
Description: Detection phosphatidylserine on the outer leaflet of the cell membrane using flow cytometry. |
![]() 2-Fluorodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0300 | Scientific Laboratory Supplies | 1ML | EUR 540.52 |
![]() 4-Fluorodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0301 | Scientific Laboratory Supplies | 1ML | EUR 540.52 |
![]() PAH Std 9-Fluorobenzo(k)fluoranthene 100ug/mL in Toluene |
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REPAH9016-I | Scientific Laboratory Supplies | 1ML | EUR 1373.49 |
![]() VOC Int Std Fluorobenzene 2000ug/mL in MeOH |
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REVOC001-I | Scientific Laboratory Supplies | 1ML | EUR 160.59 |
![]() VOC Int Std Fluorobenzene 1000ug/mL in MeOH |
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REVOC002-I | Scientific Laboratory Supplies | 1ML | EUR 124.03 |
![]() VOC Int Std Fluorobenzene 4000ug/mL in MeOH |
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REVOC003-I | Scientific Laboratory Supplies | 1ML | EUR 62.54 |
![]() PAH Std 1-Fluoropyrene 100ug/mL in Toluene |
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REPAH9012-I | Scientific Laboratory Supplies | 1ML | EUR 365.57 |
![]() PAH Std 2-Fluorofluorene 100ug/mL in Toluene |
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REPAH9004-I | Scientific Laboratory Supplies | 1ML | EUR 130.56 |
![]() PAH Std 1-Fluorochrysene 100ug/mL in Toluene |
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REPAH9013-I | Scientific Laboratory Supplies | 1ML | EUR 641.05 |
![]() PAH Std 3-Fluorochrysene 100ug/mL in Toluene |
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REPAH9014-I | Scientific Laboratory Supplies | 1ML | EUR 318.57 |
![]() PI Reagent (50μg/mL) |
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E-CK-A161-100T | Elabscience Biotech | 100T | EUR 15 |
![]() PI Reagent (50μg/mL) |
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E-CK-A161-200T | Elabscience Biotech | 200T | EUR 25 |
![]() PI Reagent (50μg/mL) |
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E-CK-A161-500T | Elabscience Biotech | 500T | EUR 55 |
![]() PI Reagent (50μg/mL) |
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E-CK-A161-50T | Elabscience Biotech | 50T | EUR 10 |
![]() PAH Std 2-Fluorophenanthrene 100ug/mL in Toluene |
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REPAH9007-I | Scientific Laboratory Supplies | 1ML | EUR 318.57 |
![]() PAH Std 3-Fluorophenanthrene 100ug/mL in Toluene |
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REPAH9008-I | Scientific Laboratory Supplies | 1ML | EUR 318.57 |
![]() PAH Std 4-Fluorophenanthrene 100ug/mL in Toluene |
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REPAH9009-I | Scientific Laboratory Supplies | 1ML | EUR 494.82 |
![]() PAH Std 3-Fluorofluoranthene 100ug/mL in Toluene |
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REPAH9011-I | Scientific Laboratory Supplies | 1ML | EUR 130.56 |
![]() PAH Std Benzo(b)fluoranthene 2000ug/ml in MeOH |
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REPAH145 | Scientific Laboratory Supplies | 1ML | EUR 160.59 |
![]() PAH Std 5-Fluoroacenaphthylene 100ug/mL in Toluene |
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REPAH9002-I | Scientific Laboratory Supplies | 1ML | EUR 445.21 |
![]() PAH Std 4-Fluorodiphenylmethane 100ug/mL in Toluene |
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REPAH9003-I | Scientific Laboratory Supplies | 1ML | EUR 168.42 |
![]() PAH Std 2-Fluorodiphenylmethane 100ug/mL in Toluene |
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REPAH9005-I | Scientific Laboratory Supplies | 1ML | EUR 168.42 |
![]() PAH Std 2-Fluorodibenzothiophene 100ug/mL in Toluene |
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REPAH7003 | Scientific Laboratory Supplies | 1ML | EUR 983.12 |
![]() PAH Std Benzo(k)fluoranthene 2000ug/ml in Toluene |
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REPAH1141 | Scientific Laboratory Supplies | 1ML | EUR 147.53 |
![]() PAH Std Benzo(a)fluoranthene 200ug/mL in Toluene |
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REPAH2401 | Scientific Laboratory Supplies | 1ML | EUR 1143.71 |
![]() PAH Std Benzo(k)fluoroanthene 2000ug/mL in Toluene |
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REPAH114 | Scientific Laboratory Supplies | 1ML | EUR 59.67 |
![]() PAH Std Benzo(ghi)fluoranthene 200ug/mL in Toluene |
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REPAH2402 | Scientific Laboratory Supplies | 1ML | EUR 1423.1 |
![]() PAH Std Dibenzo(ae)fluoranthene 200ug/mL in Toluene |
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REPAH2502 | Scientific Laboratory Supplies | 1ML | EUR 1869.62 |
![]() PAH Std 7H-Benzo(c)fluorene 200ug/mL in Toluene |
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REPAH4104 | Scientific Laboratory Supplies | 1ML | EUR 1065.37 |
![]() PAH Std 11H-Benzo(a)fluorene 1000ug/mL in Toluene |
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REPAH4101 | Scientific Laboratory Supplies | 1ML | EUR 736.36 |
![]() PAH Std 11H-Benzo(b)fluorene 200ug/mL in Toluene |
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REPAH4103 | Scientific Laboratory Supplies | 1ML | EUR 485.68 |
![]() PAH Std Benzo(b)fluoranthene-d12 100ug/mL in Toluene |
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REPAH1104 | Scientific Laboratory Supplies | 1ML | EUR 365.57 |
![]() PAH Std Benzo(k)fluoranthene-d12 100ug/mL in Toluene |
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REPAH1105 | Scientific Laboratory Supplies | 1ML | EUR 365.57 |
![]() PAH Std Indeno(123-cd)fluoranthene 200ug/mL in Toluene |
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REPAH2506 | Scientific Laboratory Supplies | 1ML | EUR 1423.1 |
![]() PAH Std Naphtho(12-b)fluoranthene 200ug/mL in Toluene |
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REPAH2601 | Scientific Laboratory Supplies | 1ML | EUR 3719.65 |
![]() PAH Std Naphtho(12-k)fluoranthene 200ug/mL in Toluene |
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REPAH2602 | Scientific Laboratory Supplies | 1ML | EUR 1423.1 |
![]() PAH Std Naphtho(23-b)fluoranthene 200ug/mL in Toluene |
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REPAH2603 | Scientific Laboratory Supplies | 1ML | EUR 2417.97 |
![]() PAH Std Naphtho(23-j)fluoranthene 200ug/mL in Toluene |
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REPAH2604 | Scientific Laboratory Supplies | 1ML | EUR 3719.65 |
![]() PAH Std Naphtho(23-k)fluoranthene 200ug/mL in Toluene |
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REPAH2605 | Scientific Laboratory Supplies | 1ML | EUR 987.03 |
![]() 3-Fluoro-24-dibromodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0305 | Scientific Laboratory Supplies | 1ML | EUR 1014.45 |
![]() 3-Fluoro-34-dibromodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0306 | Scientific Laboratory Supplies | 1ML | EUR 1014.45 |
![]() 2-Fluoro-244-tribromodiphenyl Ether 50ug/mL in Toluene |
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REPBDE0309 | Scientific Laboratory Supplies | 1ML | EUR 1014.45 |
![]() 50 ML AMPHOTERICIN B, LIQUID 250 µG/ML SOLUBILIZED |
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30-003-CF | CORNING | 50 mL/pk | EUR 97.2 |
Description: Media Catalog; Cell Culture Reagents |
![]() 50 ML KANAMYCIN SULFATE, LIQUID, 5,000 µG/ML SOLUTION |
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30-006-CF | CORNING | 50 mL/pk | EUR 117.6 |
Description: Media Catalog; Cell Culture Reagents |
![]() 4-Fluoro-234-tribromodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0307 | Scientific Laboratory Supplies | 1ML | EUR 1321.27 |
![]() 4-Fluoro-236-tribromodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0308 | Scientific Laboratory Supplies | 1ML | EUR 836.89 |
![]() 2-Fluoro-244-tribromodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0310 | Scientific Laboratory Supplies | 1ML | EUR 1014.45 |
![]() 6-Fluoro-2244-tetrabromodiphenyl Ether 50ug/mL in Toluene |
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REPBDE0311 | Scientific Laboratory Supplies | 1ML | EUR 1047.09 |
![]() 3-Fluoro-22446-pentabromodiphenyl Ether 50ug/mL in Toluene |
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REPBDE0319 | Scientific Laboratory Supplies | 1ML | EUR 1463.58 |
![]() 4-Fluoro-233456-hexabromodiphenyl Ether 50ug/mL in Toluene |
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REPBDE0323 | Scientific Laboratory Supplies | 1ML | EUR 2009.32 |
![]() 6-Fluoro-2244-tetrabromodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0312 | Scientific Laboratory Supplies | 1ML | EUR 1047.09 |
![]() 6-Fluoro-2344-tetrabromodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0315 | Scientific Laboratory Supplies | 1ML | EUR 1342.16 |
![]() 4-Fluoro-2346-tetrabromodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0316 | Scientific Laboratory Supplies | 1ML | EUR 1342.16 |
![]() PAH Std 9-Fluoro-5-methylchrysene 50ug/mL in Isooctane |
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REPAH9015-I | Scientific Laboratory Supplies | 1ML | EUR 1245.54 |
![]() 3-Fluoro-22446-pentabromodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0320 | Scientific Laboratory Supplies | 1ML | EUR 1463.58 |
![]() 3-Fluoro-23446-pentabromodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0321 | Scientific Laboratory Supplies | 1ML | EUR 1297.77 |
![]() 3-Fluoro-2244556-heptabromodiphenyl Ether 50ug/mL in Toluene |
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REPBDE0324 | Scientific Laboratory Supplies | 1ML | EUR 2009.32 |
![]() 4-Fluoro-223345566-nonabromodiphenyl Ether 50ug/mL in Toluene |
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REPBDE0327 | Scientific Laboratory Supplies | 1ML | EUR 1383.94 |
![]() Sodium Fluoride, 1 M, 100 ML |
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M-CSS-290 | MiTeGen | 100 ml | EUR 94 |
Description: Sodium Fluoride, 1 M, 100 ML |
![]() 4-Fluoro-223345566-nonabromodiphenyl Ether 50ug/mL in Isooctane |
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REPBDE0326 | Scientific Laboratory Supplies | 1ML | EUR 1383.94 |
![]() PAH Std 2-Fluoro-6-methylnaphthalene 100ug/mL in Isooctane |
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REPAH9001-I | Scientific Laboratory Supplies | 1ML | EUR 73.11 |
![]() Latex-in-a-Box Conjugation Kit, Blue 0.3 micron, 7 mL |
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LIB03-B007 | BioAssayWorks | 7 mL | EUR 880.73 |
Description: Latex-in-a-Box Conjugation Kit conjugates blue 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein. |
![]() Latex-in-a-Box Conjugation Kit, Red 0.3 micron, 7 mL |
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LIB03-R007 | BioAssayWorks | 7 mL | EUR 880.73 |
Description: Latex-in-a-Box Conjugation Kit conjugates red 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein. |
![]() Amphotericin B (Fungizone) Sol, 250 µg/mL |
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CCM1120-100 | Bio Basic | 100 mL | EUR 132.28 |
![]() Lasalocid A sodium salt Solution, ~100 µg/ml in acetonitrile |
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GW0266-1 | Glentham Life Sciences | 1 | EUR 126.2 |
![]() Lasalocid A sodium salt Solution, ~100 µg/ml in acetonitrile |
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GW0266-2500 | Glentham Life Sciences | 2500 | EUR 196.1 |
![]() PAH Std 3-Fluoro-6-methylphenanthrene 50ug/mL in Isooctane |
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REPAH9010-I | Scientific Laboratory Supplies | 1ML | EUR 1218.12 |
![]() Fluorescent Mounting Medium, 18 ml |
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E18-18 | IHC World | - | EUR 98 |
![]() Ammonium Fluoride, 10 M, 100 ML |
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M-CSS-134 | MiTeGen | 4 x 25 ml | EUR 120 |
Description: Ammonium Fluoride, 10 M, 100 ML |
![]() Fluorescent colour microtube 0.5 mL |
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DD45423 | Scientific Laboratory Supplies | PK1000 | EUR 99.18 |
![]() PAH Std 5-Fluoro-23-dimethylbenzothiophene 100ug/mL in Isooctane |
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REPAH7002 | Scientific Laboratory Supplies | 1ML | EUR 983.12 |
![]() VOC Int Std 2-Bromo-1Chloropropane Fluorobenzene 2000ug/mL in MeOH |
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REVOC007-I | Scientific Laboratory Supplies | 1ML | EUR 110.98 |
![]() 1000 ug/mL Fluometuron in Acetonitrile |
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LCS-2026 | Scientific Laboratory Supplies | 1ML | EUR 174 |